Traditional Chinese Medicine Fuan And Anti-inflammatory Pathway For The Treatment Of Postoperative Ileus In Rat

Objective: Postoperative ileus model in rat to study Traditional Chinese medicineFuan improve inhibition of gastrointestinal motility and Traditional Chinese medicineFuan Effect to the vagus nerve-cholinergic anti-inflammatory pathway.Methods:50SD rats were randomly divided into5groups(n=10).1.normal group:not surgery and intragastric administration. Abdominal surgery+NS group: two hoursafter surgery in the abdomen to perfused saline3ml. The abdominal surgery+FuanGroup: abdomen two hours after to perfused Fuan3ml. The abdominal surgery+vagusnerve transection+NS group: abdominal surgery after three days the vagus nervedisarticulation, after2hours in abdomen perfused saline3ml. Abdominal surgery+vagus nerve transection+Fuan Group: abdominal surgery after three days the vagusnerve disarticulation, after2hours in the abdomen to perfused Fuan3ml.Using thephenol red Quantitative improved method and the phenol red emptying methoddetermination gastric emptying rates and intestinal propulsion rates of rats. Carotidartery blood measured before the rats were killed and determination the expressionlevels of MCP-1and TNF-α.Results:1.Abdominal surgery+NS group (41.58±1.80%)postoperative24h intestinalpropulsion rate was significantly lower (P <0.05),comparing to normal group(87.69±2.62%).abdominal surgery+fuan group(49.45±1.27%) postoperative24hintestinal propulsion rate was significantly higher (P<0.05), comparing to abdominalsurgery+NS group.(41.58±1.80%).abdominal surgery+Vagus nerve transection+NSgroup (41.31±2.69%)postoperative24h small intestine propulsion rate was notsignificantly change (P>0.05), comparing to abdominal surgery+NSgroup(41.58±1.80%).abdominal surgery+Vagus nerve transection+fuangroup(55.69±3.31%) postoperative24h intestinal propulsion rate was significantly higher (P <0.05), comparing to abdominal surgery+vagus nerve transection+NSgroup(41.58±1.80%). abdominal surgery+vagus nerve transection+fuangroup(55.69±3.31%) postoperative24h intestinal propulsive rate was higher rates (P<0.05),comparing to abdominal surgery+fuan Groups(49.45±1.27%).2.Abdominal surgery+NS group(1.13±0.09) postoperative24h gastric residualrate was significantly higher (P <0.05),comparing to normal group(0.32±0.03).abdominal surgery+fuan group(0.61±0.02) postoperative24h gastric residual rate wassignificantly lower (P<0.05), comparing to abdominal surgery+NSgroup(1.13±0.09).abdominal surgery+Vagus nerve transection+NS group(1.13±0.09)postoperative24h gastric residual rate was not significantly change (P>0.05),comparing to abdominal surgery+NS group(1.13±0.09).abdominal surgery+Vagusnerve transection+fuan group(0.54±0.02) postoperative24h gastric residual rate wassignificantly lower (P <0.05), comparing to abdominal surgery+vagus nerve transection+NS group(1.13±0.09). abdominal surgery+vagus nerve transection+fuangroup(0.54±0.02) postoperative24h gastric residual rate was lower(P <0.05),comparingto abdominal surgery+fuan Group(0.61±0.02).3.Abdominal surgery+NS group(1216±131.03pg/ml) postoperative24h the serumTNF-α level was significantly higher(P<0.05),comparing to normal group(46.51±8.29pg/ml). abdominal surgery+fuan group (533.05±64.91pg/ml) postoperative24h the serum TNF-α level was significantly lower (P<0.05), comparing to abdominalsurgery+NS group(1216±131.03pg/ml).abdominal surgery+Vagus nerve transection+NS group(1561.36±108.79pg/ml) postoperative24h the serum TNF-α level wassignificantly higher (P>0.05), comparing to abdominal surgery+NSgroup(1216±131.03pg/ml).abdominal surgery+Vagus nerve transection+fuangroup(443.59±49.68pg/ml) postoperative24h the serum TNF-α level was significantlylower (P <0.05), comparing to abdominal surgery+vagus nerve transection+NSgroup(1561.36±108.79pg/ml). abdominal surgery+vagus nerve transection+fuangroup (443.59±49.68pg/ml)postoperative24h the serum TNF-α level was lower(P<0.05),comparing to abdominal surgery+fuan Groups(533.05±64.91pg/ml).4.Abdominal surgery+NS group(28612.74±2279.37pg/ml) postoperative24h theserum MCP-1level was significantly higher (P <0.05),comparing to normalgroup(14438.27±984.96pg/ml).Abdominalsurgery+fuan group(38387.21±2202.04pg/ml)postoperative24h the serum MCP-1level was significantly higher (P<0.05), comparingto abdominal surgery+NS group(28612.74±2279.37pg/ml).abdominal surgery+Vagus nerve transection+NS group(38715.95±2674.61pg/ml) postoperative24h the serumMCP-1level was significantly higher (P>0.05), comparing to abdominal surgery+NSgroup(27553.81±1766.77pg/ml).abdominal surgery+Vagus nerve transection+fuangroup(27553.81±1766.77pg/ml) postoperative24h the serum MCP-1level wassignificantly lower (P <0.05), comparing to abdominal surgery+vagus nerve transection+NS group(38715.95±2674.61pg/ml). abdominal surgery+vagus nerve transection+fuan group(27553.81±1766.77pg/ml) postoperative24h the serum MCP-1level waslower(P<0.05),comparing to abdominal surgery+fuan Groups(38387.21±2202.04pg/ml.5.postoperative24h the serum TNF-α was Positive correlation with postoperative24h gastric residual rate.postoperative24h the serum MCP-1was Positive correlationwith postoperative24h gastric residual rate.postoperative24h the serum TNF-α wasnegatively correlation with postoperative24h intestinal propulsion rate.postoperative24h the serum MCP-1was negatively correlation with postoperative24h intestinalpropulsion rate.Conclusion:1.Intestinal operation significantly inhibit gastrointestinal motility, whilepromoting the release of TNF-α and MCP-1. Inflammatory mechanisms are the keymechanisms that cause postoperative ileus.2.Cholinergic anti-inflammatory pathway can inhibit the release of thepostoperative TNF-α and MCP-1.The single Cholinergic anti-inflammatory pathway forthe treatment of postoperative ileus effect not obvious.3.Fuan can improve inhibition the release of TNF-α and MCP-1. intestinalpropulsion was significantly higher and gastric residual was significantly lower,Fuangranules may significantly improve postoperative.