Appraises The Mouse Puberty Candidate Gene In Known QTL Using Haplotyple Analyzes

Puberty, influenced by both environmental and genetic factors, is a complicated biologic process involving sexual maturation and gaining ability of reproduction. Onset of puberty is triggered by reactivation of the hypothalamic-pituitary-gonadal (HPG) axis and modulated by both genetic and environmental factors. Previous studies suggested that chromosome X could harbor gene(s) regulating the pubertal onset.Before the this research’s work takes the parent through selective growth starting time difference big two kind of strain mouse C3H/HeJ and C57BL/6, produces the F7generation. Through STR, the SNP marker beacon use specificity sector similar department’s pedigree construction strategy had determined in the mouse X chromosome～1cM (physics is away from1.7Mb) with mouse cloudy gate opening related Rs13483770～Rs29055848between the burst length QTL sector.This research for further fine localization mouse X chromosome in with natural growth related candidate gene, in view of growth early C3H/HeJ strain mouse, this QTL in12candidate genes (Mir505, Atp11c, Gm7073, Gm7076, Gm14661, Gm14662, Gm5637, Mcf2, F9, Fgf13, Gm715, Sox3) the coded sequence and5’the upstream5k sequence has carried on the sequence determination. Using the Lasergene7.0software analysis sequence result, first analyzes the method through SNP haplotyple, removed2genes initially (Gm14662and F9). Latter carries on the reversed direction sequence confirmation, removed3non-difference genes (Fgf13, Gm5637, Mcf2). Similar is the mouse carries on the goal fragment before this laboratory construction’s specificity sector the heavy sequence analysis, selects4candidate genes (Mir505, Atp11c, Gm7073, Gm14661). Finally, unifies this laboratory phenotype character difference big4wild mouse strain (CM25, JS11, JS13, PD5) in above candidate gene place sequence, carries on the sequence variance analysis according to the phenotype difference characteristic. Other2candidate genes (Gm7073, Gm14661) removes one by one. This time, in this sector is locked with the mouse growth starting time related candidate gene in Atp11c and Mir505.This research’s result had determined initially2individuality growth start gene, has built the good foundation for the next its related mechanism’s research. Simultaneously is also helpful in the natural growth starts the research which the related circuit adjusts. All these work are helpful in help us to understand the growth complex character entire physiological process and the regulative mechanism.