The Study Of Mechanism And Role Of Monosodiumglutamate Which Could Cause Diabetic

ObjectiveTo explore the correlation between glutamate and diabetes through animalexperiments and clinical epidemiological investigation.Experimental study that long-termintake of monosodium glutamate (MSG) Wister rats and the control group the Wister ratsblood glucose, triglycerides, total cholesterol, insulin and glutamic acid decarboxylaseantibody (GADA) and islet cell apoptosis rate, the difference between a distinctionbetween different dose groups and MSG, study its difference, to study the effect ondiabetes and its mechanism. Clinical epidemiological investigation was done to explore therelationship between MSG intake in our daily diet and incidence of diabetes in China.Research materials and methodsAnimal experiment:40newborn males Wister rats which were randomly divided into4group according to the principle of randomization, that is, the control group, MSG lowdose group, MSG medium dose group and MSG high dose group, each group of10. Thecontrol group was given basic diet and water lavage every day. The last three groups wererespectively fed with MSG solution gavage with dosage of120mg/kg,480mg/kg,960mg/kg besides the basic diet. Test blood index every4weeks. Lavage for33weeks, end ofthe molding, kill the rats, take blood from abdominal aorta, test indicators such as serumglucose, triglyceride, total cholesterol, insulin, GADA, take fresh islet tissue to testhistology index.Clinical epidemiology investigation:200diabetic patients fromendocrinology departments of five3A hospitals and200healthy residents in Shandongprovince were randomly questionnaire investigated, use SPSS13.0statistical software toanalyze the data collected.Results1. The blood glucose of control group was (19.65±4.0)mmol/L, the blood glucose of the three groups with low, medium and high dose MSG was respectively(20.97±3.98)mmol/L,(21.81±2.89)mmol/L,(21.86±2.70)mmol/L, the differences of bloodglucose did not have statistical significance; the blood glucose of rats fed with MSG washigher than the control group, and the blood glucose rose obviously as the concentration ofthe MSG was higher, which showed that MSG could elevate blood glucose.2、The triglyceride of control group and low, median, high dose MSG group, wasrespectively (1.27±0.37)mmol/L,(1.28±0.45)mmol/L,(1.55±0.61)mmol/L,(1.62±0.39)mmol/L and similarly the total cholesterol was respectively (1.78±0.41),(1.90±0.33),(1.90±0.41),(1.98±1.98), the values of triglycerides and total cholesterol were within thenormal range, and showed no statistical significance, the triglycerides and total cholesterolof rats fed with MSG were higher than the control group, and both the triglycerides andtotal cholesterol rose obviously as the concentration of the MSG was higher, which showedthat MSG could elevate the triglycerides and total cholesterol.3. The insulin level of the control group and low,median,high MSG dose group wasrespectively (0.63±0.05)mU/L,(0.84±0.10)mU/L,(1.01±0.11)mU/L,(1.31±0.16) mU/L,the insulin levels rose significantly, and showed insulin resistance, the insulin levels hadsignificant difference among different dose groups.4、The glutamic acid decarboxylase antibody (GADA) of the control group andlow,median,high MSG dose group was respectively (12.06±0.36)ng/L,(14.55±0.57)ng/L,(19.17±0.82)ng/L,(22.08±0.87)ng/L. The serum GADA levels of rats fed with MSG rose,The GADA level was higher as the feeding dose increased, and the GADA was the mark topredict islet cell damage indirectly, the differences among groups had statisticalsignificance, suggested that the MSG might have important relationship with the GADA.5、MSG can increase the islet cell apoptosis rate, and islet cell apoptosis was the finalmechanisms of diabetes occurrence. The islet cell apoptosis rate of the control group andthe experimental group was respectively (0.56±0.03)%,(0.63±0.03)%,(0.68±0.03)%,(0.70±0.03)%.6、The epidemiology investigation showed that compared with the normal crowd,diabetic patients preferred to eat monosodium glutamate, the diabetes patients’ averagedaily intake of MSG was (2.08±1.769) g, and the normal people’s average daily intake was(1.9±0.98) g, age and gender had no statistical significance, monthly income level,preference to monosodium glutamate, average daily intake of MSG and body mass index(BMI)between diabetes patients and normal people had statistical significance. conclusionsThis study confirmed that MSG had the effect to elevate Wister rats’ blood glucose,triglycerides and total cholesterol which aslo had direct and indirect damage to islet cells ofrats and there was insulin resistance. MSG had the potential mechanism to cause thediabetes. The diabetes patients preferred to MSG and the average intake was significantlygreater than that of diabetes-free population after comprehensive analysis of variousfactors, the occurrence of diabetes was associated with MSG intake and the preferencedegree to MSG.