Research On Mitochondrial Fusion Protein Expression Of Human Lung Adenocarcinoma A549Stem Cell Spheres And A549Cell Lines

Lung cancer is one of the malignant tumors. It seriously threaten the humanhealth. Previous studies show that cancer stem cells have stronger ability of resistanceto apoptosis,and play an important role in tumor formation, recurrence and malignantmetastasis and chemotherapy resistance. Mitochondria are major organ of cellapoptosis, and play a key role in apoptosis. Mitochondrial constantly undergoingfusion and divide; Studies have shown that mitochondrial fusion can strengthen thecell apoptosis resistance;there is a protective effect on the normal cells,on the tumorcells with resistance to apoptosis. But whether mitochondria fusion involved in thecancer stem cell resistance mechanisms or not,and there are no related reports aboutthem.Objective: To investigate mitochondria fusion protein expression of human lungadenocarcinoma A549cell spheres is different from A549tumor cell lines.Methods: Use the method of serum free medium (serum-free medium, SFM) tocultivate tumor stem cells of human lung adenocarcinoma A549cells, then inducedA549cell sphere formation; At last, use RT-PCR and Western blotting methods.Mitochondria fusion protein (Mfn1, Mfn2, OPA1) expression level were comparedwith the human lung adenocarcinoma A549tumor stem cells spheres and A549cellline.Results: The expression of Mfn1mRNA and Mfn1protein in A549stem cellsand in A549cell lines were respectively0.16±0.04and0.29±0.04(P<0.05).Theexpression of Mfn2mRNA and Mfn2protein in the two groups were respectively0.16±0.03and0.28±0.09(P<0.05).The expression of OPA1mRNA and OPA1proteinin the two groups were respectively0.13±0.04and0.39±0.05(P<0.05).Conclusion:1.The mitochondria fusion protein (Mfn1, Mfn2, OPA1) expressionin Human lung adenocarcinoma A549cancer stem cell spheres were stronger thanA549cell lines.2. presumably, mitochondria fusion process of human lung adenocar-cinoma A549cell spheres may be involved in resistance mechanisms that contribute to cancerstem cells.