Effect Of Silent Information Regulator1Deacetylating Nuclear Transcription Factors-κB On Myocardial Ischemia Preconditioning Alleviating Inflammatory Response

Objective To investigate the changes of SIRT content in myocardialischemia preconditioning and in myocardial ischemia reperfusion, SIRTacetylation regulation on NF-κB and inflammatory response of myocardialcell, so as to study the acetylation enzyme SIRT protection in thepreconditioning of rat myocardial ischemia reperfusion and its relationshipwith the nuclear transcription factors-κB associated InflammatoryResponse induced by myocardial ischemia reperfusion.Methods48Male SD rats, by means of random digital watch law, wererandomly divided into5groups of (n=12): sham group (group S), ischemiareperfusion group (group I/R), ischemia preconditioning group (group IPC),ischemia preconditioning+SIRT inhibitors group (group IPC+ex527) andischemia reperfusion+SIRT inhibitors group (group I/R+ex527). Themyocardial I/R injury and IPC model in rats was established by ligating andloosening left anterior descending coronary artery. Hemodynamic effectswere recorded respectively before ischemia (base line) and reperfusion120min. Spectrophotometry was used to detect the levels of LDH, CK-MB in Myocardial tissue. The activity of SIRT was detected by ELISA. Theexpression of SIRT was determined by Western blot analysis. Seruminflammation factors TNF-α, IL-6level was measured by ELISA, relativeexpression of SIRT by Western protein imprinting detection and the NF-κBp65acetylation level.Results Compared with the group S, group I/R120min reperfusionLDH and CK-MB were rising (P<0.05); IL-6, TNF-α in serum were rising(P<0.05); relative expression of SIRT was rising (P<0.05); acetylation P65was rising (P<0.05). Compared with group I/R, group IPC in120minreperfusion LDH and CK-MB were dropping (P<0.05); IL-6, TNF-α inserum were dropping (P<0.05); relative expression of SIRT was dropping(P<0.05); acetylation P65was both dropping (P<0.05). Compared withgroup IPC, in group IPC+ex527and group I/R+ex527,120min reperfusionLDH and CK-MB were dropping (P<0.05); IL-6, TNF-α in serum weredropping (P<0.05); relative expression of SIRT1was rising (P<0.05);acetylation P65was dropping (P<0.05). Compared with group IPC, relativeexpression of SIRT was no significant difference in group IPC+ex527andgroup I/R+ex527(P>0.05).Conclusion SIRT participate in the protection of ischemia preconditioning on rats myocardial ischemia reperfusion, the mechanismmay be related to the SIRT acetylation regulation on NF-κB.