Genetic Polymorphism Of IL-17A And STAT3Associated With Susceptibility To Kawasaki Disease

Objective：To summarize the clinical data of KD，and to investigatethe association between susceptibility to Kawasaki disease (KD) andpolymorphisms of both the interleukin17A gene (IL-17A) and the signaltransducer and activator of transcription gene (STAT3) in Han children inChongqing China.Methods： The clinical and laboratory data of KD in Han children inChongqing from2011to2012were retrospectively analyzed. The singlenucleotide polymorphisms of IL-17A (-197G/A,-692C/T) and STAT3(rs2293152C/G, rs957970C/T) in130patients with KD and147healthycontrol subjects were detected by sequence specific primers-polymerasechain reaction (SSP-PCR), and their differences were analyzed.Results：1、Occurrence rate of KD-CAL+was40.8%; The PLT and ESR inKD-CAL+were markedly increased than their in KD-CAL-〔PLT(×109/L)420.9±89.2vs355.2±68.2，ESR（mm/h）55.3±19.2vs39.8±10.2，P<0.05〕, and there were no significant difference between the two groups for WBC, CRP, Hb, Alb and LDH（P>0.05）; Occurrence rate ofIVIG-resistant KD was8.5%; The Hb and Alb in the IVIG-resistant KDpatients were decline sharply compared with which in the IVIG-sensitive〔Hb（g/L）109.4±11.3vs91.6±8.5，Alb（g/L）36.7±3.6vs23.5±2.3，P<0.05〕, and there were no statistic differences between them forWBC, CRP, PLT, ESR and LDH（P>0.05）.2、The polymorphisms of-197G/A in IL-17A：The frequencies ofAA genotype at-197G/A in IL-17A in KD patients (29.2%) wassignificantly higher than that in controls (15.0%)(P=0.004), and childrenwith A allele were1.412times more likely to suffer KD than children with Gallele (95%confidence interval1.009-1.975, P=0.044).3、The polymorphisms of-692C/T in IL-17A：There were no statisticdiffere nces in frequencies of genotype (CC、 CT、 T T) and allele (C、T) at-626in IL-17A between patients and controls (P>0.05).4、 The polymorphisms of rs2293152C/G in STAT3：The frequenciesof C allele and its homozygous CC genotype at rs2293152C/G in STAT3inKD patients were significantly higher than that in controls (P=0.006andP=0.002, respectively).5、 The polymorphisms of rs957970C/T in STAT3： There were nosignificant differences in frequencies of genotype (CC、 CT、 TT) and allele(C、T) at rs957970in STAT3between patients and controls (P>0.05). 6、There were no markedly differences in frequencies of genotype andallele between the KD-CAL+and KD-CAL-in IL-17A（-197G/A、-692C/T）and STAT3（rs2293152C/G、rs957970C/T）; There were nostatistic differences could be found in data of laboratory test (WBC、Hb、PLT、CRP、ESR、Alb、LDH) between the genotype（P>0.05）.Conclusions：1、The prevalence of KD-CAL+was40.8%and the IVIG-resistant KDwas8.5%in this study. The higher PLT and ESR were risk factors forKD-CAL+. The dramatic decline of Hb or Alb may be an alarm of IVIG-resistant in KD patients.2、The results indicated that the IL-17A gene promoterpolymorphisms at position-197G/A confered susceptibility to KD in Hanchildren in Chongqing China. The children with A allele were morevulnerable to KD. There were no significant correlation betweenpolymorphisms of I-692C/T in L-17A gene and susceptibility to KD.3、This study suggested that the association between STAT3genepolymorphisms at position rs2293152C/G and susceptibility to KD in Hanchildren in Chongqing China, and with C allele were more susceptibility toKD. G allele may play a role in preventing KD. But STAT3genepolymorphisms at position rs957970were possibly not related with KD inHan children in Chongqing China.