Novel Cross Talk Between CXCR4and NMDAR1in EF Induced Migration Of Rat’s Neuronal Stem Cells

Objective To investigate the effects of endogenous electrical field (EF,250mv/mm) on the expression of CXCR4and NMDAR1, and cross talk between each other in neural stem cells (NSCs) of rat, and the possible mechanism.Methods The isolated rat NSCs were cultured and exposed to EF. Co-immunoprecipitation and Immunocytochemistry were used to investigate the cross talk between CXCR4and NMDAR1in rat NSCs after EF stimulaion. The expressions of CXCR4and NMDAR1in rat NSCs after EF stimulation and the possible downstream signal pathway were assessed with Western Blots.Results Here we showed that EF could increase the expression of NMDARl and CXCR4with a time-dependent relationship. Interestingly, EFs could induce the cross talk between CXCR4and NMDAR1with a time-dependent form. In addition, EF could include sustained phosphorylation of Akt and corresponding dephosphorylation PTEN implicated in directing NSCs migration.Conclusions In the present study, we showed for the first time that EF could induce the cross talk between NMDAR1and CXCR4. Our work first confirmed that EF could induce NMDAR1/CXCR4interaction in directing NSCs migration, which might through the PTEN/PI3K/Akt signaling pathway. Our findings suggest that EF drive NSCs migration may though a novel cross talk between chemotaxis receptors and neurotransmitter receptors, which provides a new treatment strategy for nerve damage and repair.