To Discuss The Effect Of High Intensity Focused Ultrasound To Skeletal Growth In The Human Neuroblastoma’s Model In Infancy SD Rats

PART1:ESTABLISHMENT OF HUMAN NEUROBLASTOMA’MODEL IN INFANCY SD RATSObjective:To establish human neuroblastoma model and explore its feasibility in infancy SD rats.Methods:Human neuroblastoma cell (Cell line:SK-N-SH) was culture in vitro.(1-3)×107/ml cells in0.2ml of suspension were collected and inoculated into the capsule sac of the left adrenal gland using syring in SD mouse. The volume of tumor was investigated using ultrasonograph, and the growth cruve was drawn, furthermore, specific marker NSE of neuroblastoma was investigated by using immunohistochemistry assay.Results:Tumor successfully grew in all35injected SD rats(100percent of tumor formation rate), and3cases of cachexia occurred due to metastasis. The tumor tissue grew locally in other tat. The tumor growth cruve showed that growth peak occurred on the9th day after injection and reduced in volume on around17th day. Tumor tissue in2cases regressed in experimental group after1month, others regress after3months. Positive NSE expression could be seen in all animal.Conclusion:Human neuroblastoma model is available to be established to be established in infancy SD mouse with higher foemation rate in tumor tissue, which can be ultilized to simulate and study their biological characteristics involving their immune mechanism in vivo. It is also ideally experimental model for studying physical therapy of neuroblastoma in economy and practice. PART2:TO DISCUSS THE EFFECT OF HIGH INTENSITY FOCUSED ULTRASOUND TO SKELETAL GROWTH IN THE HUMAN NEUROBLASTOMA’S MODEL IN INFANCY SD RATSObjective:To discuss the effect of the skeletal growth in the human neuroblastoma’s model in infancy SD rats by comparing normal SD rats with different energy gradients of HIFU (High intensity focused ultrasound) treatment for human neuroblastoma’s model in infancy SD rats.Methods:904-week-old SD rats were divided into2group.Each group had45rats.Group A was normal group,Group B was HIFU group, Human neuroblastoma cell (Cell line SK-N-SH) was collected and inoculated into the capsule sac of the left adrenal gland using syringe in SD mouse,then different energy gradients of HIFU treatment for them.The length and malformation of SD rats,and the spine and ribs around HIFU focused area pathology comparison,and the level of serum bone alkaline phosphatase(BALP) by enzyme league immune method (ELISA) at HIFU treatment3days,2weeks and4weeks.Results:Malformation was not found by macroscopic observation. X-ray examination found that only one human neuroblastoma’s model in infancy SD mouse got spina bifida at4weeks after HIFU treatment. There were significant differences in the height growth of SD rats and the level of serum BALP between3days and2weeks after HIFU treatment(P<0.05).No significant differences in them during4weeks after HIFU treatment (P>0.05). Hispathology showed that, compared to group A,bone trabeculae distribution sparse,uneven thickness,bone marrow cavity expansion between3days and2weeks after HIFU treatment.Bone trabeculae degree are consistent,uniform distribution both in the2group during4weeks after HIFU treatment.Conclusion:HIFU may inhibit skeletal growth of the human neuroblastoma’s model in infancy SD rats in short time after HIFU treatment,but the inhibit effect can be disappear during adolescence.