Study On The Efficacy And Hepatotoxicity Of Tripterygium Wilfordii Compound Recipe GEL On Adiuvant Arthritis In Rats

Objective：To discuss the treatment effect and the impact on liver function andreproductive system of different doses Tripterygium wilfordii compoundrecipe gel (TWCG) for external use on adjuvant arthritis(AA) in rats.Methods：Rats were percutaneous administrated with homemade different dosesof TWCG after the freund’s adjuvant arthritis model was established. Thegambrel swelling degrees were evaluated;the serum levels of tumor necrosisfactor-α（TNF-α） and interleukin-4（IL-4）were detected by ELISA;thelevels of matrix metalloproteinase-3（MMP-3）in ankle joint cartilagewere determined by immunohistochemical method; The serum levels ofalanine aminotransferase（ALT），aspertate aminotrans-ferase（AST）were tested with the automatic biochemistry analyzer to observe thehepatotoxicity; the levels of vascular endothelial growth factor（V EGF）inovary were determined by immunohistochemical method to observe the reproduction toxicity.Results：The gambrel swelling degree in TWCG groups and voltaren group weresignificantly reduced（P<0.05）；the levels of TNF-α in TWCG groupsand voltaren group were visibly decreased and IL-4were visiblyincreased（P<0.05）；the content of MMP-3in ankle joint wassignificantly reduced. Effects in TWCG high dose group and median dosegroup were better than voltaren group; however，the differences of ALT andAST were not statistically significant among the groups;the expressionlevels of VEGF in rats ovaries had no statistical difference among thegroups.Conclusion:TWCG had obvious anti-inflammatory and detumescence effect on AArat.It could protect cartilage tissue effectively, while it had no significantliver toxicity and reproduction toxicity.