Correlation Of The Elderly Hypertension Patients With Heart Brain And Kidey Target Organ Damage With Single Nucleotide Polymorphismof Of Klotho Gene

Objective: To study the associations between single nucleotidepolymorphisms (SNPs) of Klotho gene (G-395A, C370S and F352V) andtheir target organ damages in the elderly patients with essentialhypertension(EH), this study was to investigate allele distrutioncharacteristics and genotype of these SNPs in the EH hypertension patientswith target organ damage and without target organ damage in Chongqing.Methods: According to the diagnostic criteria, they were divided intohypertension with coronary heart disease group (CHD)(236cases),hypertension with cerebrovascular disease (136cases) and hypertensionwith renal damage group (208cases).Genomic DNA of the peripheralleukocytes was separated and extracted. Allele distrution characteristicsand genotype of these three SNPs were detected through this technique ofsingle allele specific primer PCR technique (SASP-PCR). Results:1. The rate of FV genotype of KL F-352V in the EH group with CHDand the EH group without CHD were59.74%and69.68%separately. Theresults showed a statistically distinctly difference (P<0.05). According togender stratification research, we found the rate of FV genotype in the maleEH group with CHD was60.29%and that in the male EH group withoutCHD was73.37%. Between these two subgroups the difference wassignificant (P<0.05). The results of the genotype distributions of KLF352VFF, VV did not showed statistically distinctly difference between twogroups(P>0.05).The results of the genotype distributions of KLG-395A SNP GG, GAand AA between two groups did not showed statistically distinctlydifference (P>0.05). But according to gender stratification research,, theresult showed that the rate of GG genotype in female subgroup patients withCHD was50.46%. The frequency of GG genotype was29.85%in the femalesubgroup patients without CHD. Contrast of the two subgroups patients,theresult showed a statistically distinctly difference (P<0.05). We also foundthat the frequency of AA genotype in the male subgroup patients with CHDwas65.85%.，the frequency of AA genotype in themale subgroup patientswithout CHD was45.45%. Contrast of the two subgroups patients, theresults showed a statistically distinctly difference (P<0.05)The results did not showed a distinctly difference in the genotype rateof KLC370S CC, CS and SS between the group patients with CHD and those without CHD (P>0.05).According to sex stratification subgroupanalysis, we did not find statistically difference between these twosubgroups (P>0.05).2. The results did not showed a distinctly difference in the genotypefrequencies of KLG-395A SNP GG, GA and AA, KL F352V SNP FF,FVand VV,and KL C370S SNP CC,CS and SS between EH withcerebrovascular disease group and EH without cerebrovascular diseasegroup (P>0.05). In gender stratification research, we did not findstatistically difference between these two subgroups (P>0.05).3. The results did not showed a distinctly difference in the genotypefrequencies of KLG-395A SNP GG, GA and AA, KL F352V SNP FF,FVand VV,and KL C370S SNP CC,CS and SS between EH with renaldamage group and EH without renal damage group (P>0.05). In sexstratification subgroup research, we did not find statistically differencebetween these two subgroups(P>0.05).Conclusions:1. This study fond that the EH patients and the male EH patients whocarried FV genotype of KL F352V SNP are not easy to infect CHD.2. This study fond that the female EH patients who carried GGgenotype of KLG-395A SNP are easy to infect CHD. This study fond thatthe male EH patients who carried AA genotype of KLG-395A SNP are easyto infect CHD. 3.This study did not find the association of SNP of KLG-395A，F352V,C370S with brain and kidney target organ damage in the EHpatients...