Effect And Mechanism Of Golgi α-mannosidase Ⅱ Expression Inhibition By RNA Interference On Invasive Potential Of Human Gastric Cancer Cell

Objective:The divergence expressions of GMⅡ in different gastric cancer celllines were analyzed to investigate the role of GMⅡ in gastric cancergenesis.Methods:The pGPU6/GFP/Neo-GM Ⅱ-1406gene was transfected intoMGC-803and SGC-7901cells using cationic liposome assay. The rates oftransfected positive cells were performed under invert microscope. Thegene and protein level expressions of GMⅡ and E-cadherin、α-cateninwere tested using RT-PCR and Western blotting assay, respectively. Theinvasive abilities of cancer cells were analyzed by cell scratch andTranswell assay.Results:The proteins expression of GMⅡwas significantly inhibited and theexpressions of E-cadherin and α-catenin in MGC-803and SGC-7901cells have been up-regulated compared with untransfected cells.(P<0.05).Cell scratch assay showed that the invasive abilities of cells transfected with GMⅡ gene were weaker than control group cells(P<0.05). Transwellinvasive assay demonstrated that the invasive abilities of cells transfectedwith GMⅡ gene were both weaker than shNC groups, the numbers oftranswell cells are（65±5），（197±6），（186±5）and （72±4），（178±4），（184±5）in three groups during18h (P<0.05).Conclusion:GMⅡgene silensceing contributes to decreased invasive ability ofgastric cancer cell lines, which is involved with up-regulation of E-cadherinand α-catenin expressions.