Factors Affecting Urinary Polycyclic Aromatic Hydrocarbons Metabolites In An Urban Adult Population In Wuhan

Part One: Levels of urinary monohydroxy polycyclic aromatichydrocarbons metabolites in the adult populationObjective The aim of this part was to measure the levels of urinary monohydroxypolycyclic aromatic hydrocarbons (OH-PAHs) metabolites in the adult population toestimate the intenal levels of PAHs in the study population.Methods The study recruited2474adult subjects who lived in Wuhan Urinary10OH-PAHs metabolites were analyzed by Gas chromatography/mass spectrometry(GC/MS) method. Urinary OH-PAHs metabolites levels were subsequently adjustedby urinary creatinine (Cr) levels. Results After adjusted by urinary creatinine levels, each component proportion oftotal OH-PAHs metabolites were32.72,27.55,28.20and11.53%for naphthalenemetabolites, fluorene metabolites, phenanthren metabolites and pyrene metabolites,respectively. The geometric level (95%confidence interval,95%CI) of2-hydroxynapthalene (2-OHNap) was11.00μg/g Cr (10.70μg/g Cr-11.32μg/g Cr,which was higher than those of the other9OH-PAHs metabolites (p <0.001).Conclusions The proportion of naphthalene metabolites of total OH-PAHsmetabolites in urine was the highest,in the4kinds of PAHs metabolites2-OHNaplevel in urine was higher than the other9OH-PAHs metabolites.Part Two: Affecting factors of urinary monohydroxy polycyclicaromatic hydrocarbons metabolitesObjective The aims of the part were to investigate factors affecting levels of urinaryOH-PAHs metabolites among an adult population and evaluate whether1-hydroxypyrene could be considered as an representative exposure marker in thestudy population.Methods We analyzed the distributions of OH-PAHs metabolites levels in thesubgroups according to the selected variables according to demographic and housecharacteristics and diet habits; Multiple logistic regression analysis was performed toanalyze the risk factors for the urinary OH-PAHs metabolites; The correlations ofurinary OH-PAHs metabolites with each other were further analyzed using the partialcorrelation analysis;Results The logistic regression analysis showed that urinary OH-PAHs metaboliteslevels among participants from Hankou district in Wuhan were higher (OR>1.00) thanin ones from Hanyang district in Wuhan (OR=1.00), expect for1-OHNap and1-OHPye; females had higher urinary OH-PAHs metabolites levels (OR>1.00)compared with males (OR=1.00), except for2-OHFle; the urinary OH-PAHs levelswere higher in the participants in the old age group (≥60years old, OR>1.00) compared with the youth age group (18-44years old, OR=1.00), except for1-OHPye;the participants who used coal and firewood as domestic fuel had higher urinaryOH-PAHs metabolites levels (OR>1.00) compared with those who used natural gas,except for2-OHNap,4-OHPhe and9-OHPhe; current smokers had higher urinary1-OHNap,2-OHNap and2-OHFle levels (OR>1.00) than non-smokers (OR=1.00).The factors affecting urinary OH-PAHs levels were different,, the positive correlationsbetween urinary OH-PAHs metablites each other were found (P <0.01).Conclusions The potential factors affecting urinary OH-PAHs levels among the studypopulation were residential district, gender, age, smoking status, cooking fuel typeand so on,. The simultaneous determination of multiple OH-PAHs metabolites mayobjectively reflect the internal levels of PAHs among the study population.