The Alterative Expression Of EPHA10Participating In The Progression Of Malignant Phenotype In Breast Cancer

Objectives: The low expression of EphA10in normal breast tissue is generally believed,but the relation with oncogenesis and lymphatic metastasis has been a matter of conjecture.The study of alterative expression of EphA10in breast cancer is addressed to show the rolein cancer malignant phenotype. Methods:1). A difference of expression of EphA10between normal breast tissue, invasive ductal carcinoma and relative metastatic lymph nodewas detected by immuno-histochemisty.2).The mRNA expression of two differenttranscript variant of EphA10(EphA10and EphA10s) were evaluated with quantitativereal-time RT-PCR, respectively.3). Design and constrction of EphA10SiRNA andpEGFP-T2A-EphA10s.4). The protein expressive change of EphA10and EphA10s wasassessed after transfected with EphA10SiRNA and pEGFP-T2A-EphA10s inMDA-MB-231cell line.5).Cell proliferation and apoptosis assay was examined throughflow cytometry (FACS) after disturbing the protein level.6). cell wound scratch assay,transwell assay and immunofluorescence was used to evaluate the change of invasion andmigration, respectively. Results:1) the expression ofEphA10in breast cancer is higherthan which in the normal ovarian, and in metastatic lymph node the expression is muchstronger.2). In cell line, the expression of EphA10and EphA10s shows an absolute diferent,as MDA-MB-231shows a higher lever in EphA10and a lower level in EphA10s.3). Theablity of migration and invasion is weaken in different level after transfected with EphA10SiRNA and/or pEGFP-T2A-EphA10s, respectively. And the same result is found though theimmunofluorescence4). The level of cell proliferation and apoptasis is little fluctuatedcompared with control. Conclusion: With the malignant progression in breast cancer, theexpression level of EphA10is intensified. And regulating the expressive level of EphA10and EphA10s could convert the ablity of migration and invasion in breast cancer cell line.