The Effect And Significance Of Glucocorticoid On CD163and Cytokines Of Acute-on-chronic Liver Failure Rats

Objective To investigate the effect of glucocorticoids on CD163andpro-inflammatory and anti-inflammatory cytokines of acute-on-chronic liver failuremodel rats.Methods30SPF male Wistar rats were randomly divided into three groups：6ones in control group,6ones in model group,18ones in treatment group. Afterintraperitoneally injected with50%CCL4olive solution for eight mouths, the rats wereintraperitoneally injected with D-galactosamine(D-gal) and lipopolysaccharide(LPS)for making the acute-on-chronic liver failure model. Rats in the treatment group weredivided into three groups according to the different times of dexamethasone treatment:0hour treatment group with dexamethasone after modeling,4hour treatment group,8hour treatment group. All rats were sacrificed at24h after modeling. Automaticbiochemical analyzer detected serum ALT, AST and TBIL. Pathological changes ofliver tissue were detected after conventional H-E dyeing. The expression of CD163inliver tissue was detected by Real-time quantitative PCR. The content of TNF-a andIL-10in the serum was examined by ELISA． Results The acute-on-chronic liver failure model rats were well prepared.Pelleta and massive necrosis was appeared based on liver fibrosis and inflammatorycells infiltrated obviously. Liver transaminase，TNF-a in the serum significantlyincreased. In the0hour treatment group with dexamethasone after modeling, livertransaminase and TNF-a were remarkably reduced comparing with the model groupwhile IL-10and the expression of CD163in liver tissue was increased（P<0.05）. Inthe4hour treatment group and8hour treatment group the above indexes were notobviously changed.Conclusions: In the early stage of acute-on-chronic liver failuredexamethasone can balance the pro-inflammatory and anti-inflammatory cytokines,and can furthermore promoted the expression of CD163for the protection of liver.