The Effect And Mechanism Of Ginsenoside Rb1on Coagulant And Fibrinolysis Activity In Hepatic Sinusoidal Obstruction Syndrome Of Rats

Objective s To construct the HSOS model of rats and conifrm that endothelial cellprotective agent ginsenoside Rbl can prevent HSOS by reducing TF and PAI-1andreversing high condensation and low ifbrinolysis state induced by monocrotaline(MCT).Method： we constructed the HSOS model of SD rats through perfusing MCT intostomach and intervened the model using the ginsenoside Rbl. The experiments weredivided as follows：①control group；②model group： MCT160mg/kg；③MR10group： MCT160mg/kg+Rbl10mg/kg；@MR20group： MCT160mg/kg+Rbl20mg/kg；?MR30group： MCT160mg/kg+Rbl30mg/kg；?Rbl10mg/kg；?Rbl20mg/kg；⑧Rbl30mg/kg. Then we tested the levels of TF and PAI-1in the plasmaand liver using ELISA，RT-PCR and Western blot and the activity of coagulant andifbrinolytic system of each SD rat in all groups.Results：①ELISA showed that in the model group, the plasma TF, PAI-1levels weresigniifcantly higher than control group, the differences were statistically signiifcant (P<0.05). In prevention group of Rbl20mg/kg, plasma TF, PAI-1levels weresigniifcantly lower than model group, the difference was statistically signiifcant (P< 0.05). While in prevention group of Rbl30mg/kg，the plasma TF was signiifcantlylower but not PAI-1. In prevention group of Rbl10mg/kg, there was no statisticallysigniifcant difference compared with model group. There was also no signiifcantdifference between the group of using Rbl alone and the control group.②The resultsof RT-PCR showed that in the model group, the mRNA levels of TF, PAI-1in theliver were signiifcantly higher than control group, the differences were statisticallysigniifcant (P<0.05); in prevention groups of Rbl20mg/kg and30mg/kg, the mRNAlevels of TF, PAI-1in the livers were signiifcantly lower than model group (P<0.05);While in prevention group of Rbl10mg/kg, there was no statistically signiifcantdifference compared with model group.③The results of western blot showed that inthe model group, the protein content of TF, PAI-1in the liver were signiifcantlyhigher than control group (P<0.05); in prevention group of Rbl20mg/kg, the proteinlevels of TF, PAI-1in the livers were signiifcantly lower than model group(P<0.05);While in prevention group of Rbl10mg/kg and Rbl30mg/kg，there was nostatistically signiifcant difference compared with model group. There was nosigniifcant differences between the groups of using Rbl alone and controlgroup.@The results of coagulant and ifbrinolytic test showed that APTT, PT, TT, ATTand D-D of MCT group were higher，while AT-A and Fbg were lower than controlgroup. In the prevention groups of Rbl，APTT，PT，TT，ATT and D-D were higher butAT-A and Fbg were lower than the MCT group.Conclusion：①Monocrotaline160mg/kg could successfully construct HSOS modelof rats.②The results further conifrmed that20mg/kg and30mg/kg of ginsenosideRbl can effectively prevent the happening of HSOS, but20mg/kg was better.③Monocrotaline may damage hepatic sinus endothelial cells and affect the activity ofcoagulant and ifbrinolytic system by increasing the TF and PAI-1, causing highcondensation and low ifbrinolysis state of hepatic sinus and leading HSOS to happen. ④The ginsenoside Rbl may effectively prevent the happening of HSOS throughprotecting hepatic sinus endothelial cells and reducing the level of TF and PAI-1.