Curcumin Against Hyperoxia-induced Damage In AECâ…¡s

Objective:We explored the protective effects of curcumin against hyperoxia-induced cell damage in primary rat alveolar epithelial type II cells from premature rat, focusing on the effect of curcumin on the regulation of the level of ROS, the Nrf2nuclear translocation and the expression of antioxidant enzyme.Method:Embryonic day19fetal rat AECIIs were cultured in vitro and divided randomly into air group, hyperoxia group and Curcumin-treated group. AECIIs in hyperoxia group were exposed to95%02/5%C0210min and subsequently were sealed. AECIIs in Curcumin-treated group were treated with10umol/L curcumin and then were exposed to95%02/5%C0210min. All groups of AECIIs were cultured in ordinary incubators (37℃,5%C02)24hrs. The cell morphological changes and growth condition were observed by the inverted phase contrast microscope; The cells viability were measured by CCK-8;The ROS level was measured by flow cytometry; Nrf2nuclear translocation were observed by immunocytochemistry; The levels of GPX2mRNA and HO-1mRNA were measured by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR).Result:Compared with the group exposed to air, the cell viability of AEC lI decreased, the production of ROS and the RT—PCR levels of HO—1and GPX2mRNA increased in the group exposed to95%02/5%CO2(P<0.05); Curcumin treatment upregulated cell viability, HO-1mRNA and GPX2mRNA on PT-PCR level, downregulated the generation of ROS, as well as increased Nrf2nuclear translocation compared with the group exposed to95%02/5%CO2(P<0.05). Conclusion:These results suggest that Curcumin reduce the level of ROS, augment Nrf2nuclear translocation, the expression of HO-1and GPX2and against hyperoxia-induced damage in AECⅡ s.