Effects Of Different Suction Hours On Viability Of Fat Granules

Objective:To confirm cell damage before transplantation of adipose cells by comparing cell breakage ratio and activity of fat granules in different suction hours, providing a theoretical basis for autologous fat granules transplant.Method:Liposuction was done using3mm blunt liposuction needle(the size of side hole is2.5mm×5mm), under the negative pressure of the20ml syringe (the piston was fixed on20ml scale marking site), and in the same tunnel of the lower abdomen of13cases. Five tubes of fat aspirates were acquired by suctioning15times per round trip. After the fat granules were cleaned and purified, HE staining were performed, then cell breakage ratio was calculated under the inverted contrast microscope. Cell activity was analyzed and compared by MTT method and glucose transportation test.Results:HE staining showed the cell breakage ratio were14.7±1.7%(the1st tube),16.2±2.1%(the2nd tube),42.2±2.7%(the3rd tube),56.2±3.2%(the4th tube), and73.2±5.2%(the5th tube) respectively. The transfer amount of glucose were0.742±0.11mmol/L (the1st tube),0.670±0.08mmol/L(the2nd tube),0.360±0.05mmol/L(the3rd tube),0.254±0.05mmol/L(the4th tube), and0.216±0.06mmol/L(the5th tube) by glucose transfer experiments respectively. The optical density(OD) value by MTT method showed that they were1.149±0.065(the1st tube),1.095±0.048(the2nd tube),0.757±0.095(the3rd tube),0.562±0.101(the4th tube),0.477±0.086(the5th tube) respectively. The above results showed no statistical difference between the1st and2nd tube (P>0.05),and a significant difference among the2nd,3rd,4th and5th tube (P<0.05). Conclude:The viability of fat granules will decrease with the increase of the number of suction. The fat granules acquired in the former period have higher viability, they are suitable for transplantation in vivo.