The Effect Of STIM1/Orai1on The Cell Proliferation In Primary SD Rat Astrocytes

Astrocytes are an important part of the nervous system, whose content is more than80%in brain tissue and function is also very widely. It is involved in the blood-brainbarrier formation, the blood-retinal barrier composition, and in the process ofdifferentiation of the endothelial cells regulative effect. Meanwhile, astrocytes are ableto support the growth of neurons, which is especially related to hippocampal neurons.Fourthermore, astrocytes also play important roles in the regulation of neurotransmittersrelease, pass, synapses and memory formation. Above all, calcium and calcium channelare involved in and occupy an crucial role. With the discovery of STIM/Orai and depthstudy of the SOCE, the effect of them on cell cycle is also growing concerned. Severalinhibiters of calcium channel which are used in the study of SOCE such as Verapamil,TH-1177,2-APB, SK&F96365and CAI are shown to inhibit cell proliferation. Gd³⁺asa common tools drug which used in the study of calcium channel can inhibit a variety ofcalcium channels. The present study set out to investigate the influence of STIM1,Orai1and Gd³⁺on rat primary astrocytes’ cell cycle. Using calcium imaging and flowcytometry assays, we observed that silencing STIM1or Orai1in rat primary culturedastrocytes resulted in cell cyle alteration, cells arrest at G0/G1phase, whereas G2/Mphase was significantly reduced. shSTIM1and shOrai1exhibited similar efficacy onastrocytes’ cell cycle arrest.