Effect Of Amino Acids On Lifespan Of C. Elegans And Preliminary Study On Molecular Mechanism

Longevity is the long-cherished common wish of human being, and prolonginglifespan and delaying senescence has gradually become one of the most popularsubjects in bioscience research field currently. Regulatory mechanism of lifespan iscomplex decided by genetic gene and external environment simultaneously, andcrucially depended on by genetic genes, but without no clear mechanisms. In thispaper, Caenorhabditis elegans has been chosen as model organism to exploremolecular mechanism of lifespan longevity adjustment by means of impact on itslifespan and characteristics by using amino acid, thereby achieving the purpose ofretarding, even reversing senium by adjusting diet to prolonging lifespan.According to the literature in hand and related information collected, thelongevity-related gene of C.elegans which has been published explicitly is concludedand summarized, bioinformatics database and KGML software have been utilized tosuccessfully structure the Local gene network pattern of C.elegans. This LGNPpattern combines the related gene, small molecule compounds and metabolic moduleclosely by relevant biological pathway. This gene network surmounts the oldresearch mode of lifespan adjustment achieved by causing single gene mutation, andclearly displays the interation of genes, influence on small molecule on geneticexpression and regulating effect of relevant metabolic pathways. It is a reliabletechnology platform for exploring the mechanism of regulating molecule of lifespanfrom a new point of view.By feeding C.elegans with21kinds of amino acid small molecule in threedifferent concentrations (0.1%,1%and10%separately) and observing the influenceon lifespan of C.elegans,16kinds of groups could be screened which have anobvious noticeable influence. Base on this consequence, two parallel tests andobservations about physiological feature have been done with the consequence that10Cys,10Asn,10Thr,10Asp,1Tyr and1Met could either influence the lifespan ofC.elegans, or have an exellent experimental reproducibility, among which10Cysinfluence the physiological feature of C.elegans observably, not only improve theactivity, but also get the thiner and smaller size C.elegans by feeding which kind ofamino acid. By comprehensive consideration,10Cys,10Asn and10Asp are finallyconfirmed as the testing targets of gene level transformation.By employing real-time fluorescence quantitative PCR to detect longevity-related gene expression levels of C. elegans fed by10Cys,10Asn,10Asp for5days,10days and15days respectively, experimental results showed that an average ofdifferent degrees of changes occered on the expression level of each genes, of which gene expression levels of age-1and pdk-1genes in C. elegans fed by10Cys in thefifth day had increased more than2times compared with that fed by other aminoacid. Four genes(ie. age-1, pdk-1, akt-1, and nmr-1) in C. elegans fed by10Aspfeeding showed the characteristic that the longer feeding time it was, the higher geneexpression quantity detected, of which nmr-1in C. elegans fed by10Asp, theexpression quantity of15days was much higher than that of5days, up to tens ofthousands of times. The findings showed that amino acid could affectlongevity-related gene expression level, but obvious regularity had not been foundfrom the perspective of local gene network, further study was still required.