Photodynamic Inactivation Of Esophageal Cancer Cells And Methods Of Fluorescence Diagnosis

As a new technology, photodynamic therapy has a very broad prospects anddevelopment potential. But the application of photodynamic therapy in terms of dose isblind and random, due to the limitation of diagnosis technology. The research is carriedout on fluorescence diagnosis and fluorescence imaging, and its application to thedetection and inactivation of esophageal cells, in order to make up for the inadequacy ofthis aspect.With hematoporphyrin monomethyl ether (HMME) be the object of detection,research is carried out on fluorescence diagnosis methods, the optical properties ofHMME is detected and analyzed, using spectroscopy detection analysis. The energylevel diagram of HMME is mapped, and the mechanism action of fluorescencespectrum mediated by HMME is analyzed, based on the theory of atomic physics. Thedevice for fluorescence spectrum diagnosis is designed and settled, fluorescence ratiomethod theory model is established, the detection limit of this fluorescence diagnosissystem is6μg/L, and it is successfully applied to the diagnosis of the metabolism ofHMME in the esophageal cancer cells.The research is carried out on how the optical parameters (de nsity of HMME andtime for irradiation) impact the inactivated effect of esophageal cancer cells. In thephotodynamic action, the relation of esophageal cancer cells survival and opticalparameters is close to e index.In the study of two-dime nsional fluorescence imaging method, the2-dfluorescence imaging device is designed and settled, the theoretical equation for2-dfluorescence ratio imaging methods is deduced according to the theory of thefluorescence ratio method model. The signal-to-noise ratio is improved by using themethod of multiple pixel point addition and trying to increase the inte nsity of eachpixel unit. The detection limit for the photose nsitizer of this2-d fluorescence imagingsystem is1.9μg/L, contrast degrees is improved from0.4to0.8after processed. Thesystem is successfully applied in the2-d image without background of HMME.