Development Of A Novel Bioreactor Capable Of Mechanical Stimulation And The Study For Cells Culture On Tissue-engineered Scaffolds

Tissue engineering which utilizes the principle and method of engineering andLife Science aims to study the formation and regeneration of body tissues/organsand construct a substitute with biological activity, which is embedded in the body soas to repair or improve part or the whole of the human body tissue/organ function.This paper focus on the development of new mechanical load bioreactor and itsapplication in order to study the influence of mechanical stimulus on the growth ofthe cells under the three-dimensional scaffolds in vitro. With the aim to make cellsin vitro proliferation, differentiation, and increase the mechanical properties ofcell/scaffold structures, It is of great significance for the construction of tissuesubstitutes, guiding clinical diagnosis and treatment, improving the level of humanhealth and economic development.Firstly, a kind of new bioreactor which can provide mechanical stimulation tocells in vitro is developed in this paper. Taking the special environmentalrequirements for cells culture into consideration, this paper designs the structurefrom the following four aspects: the piezoelectric ceramic dynamic compressionloading, stepper motor dynamic shear loading, the differential screw mechanism offine-tuning capabilities, and can be used for training material elastic modulus. Inorder to verify the rationality and feasibility of device structure, the wholestructure；the differential screw mechanism and the pure bottom parts based on finiteelements are respectively analyzed based on the software ANSYS WORKBENCH.Secondly, the mechanics testing model device is established. Based on thismodel, the Data Collecting System was designed and the dynamic apparent elasticmodulus was tested and analyzed. The strain gauge was applied to simplify theexperimental system and in contrast of the Data Collecting System, five kinds ofmechanical loading modes for cells culture are finally designed.In the aspect of cell culture, the collagen was used to do surface modificationon PLLA scaffolds, then the analysis of scaffold modified results were done throughcell compatibility experiment. The osteoblasts, cultured in the vitro3-dementionalscaffolds, was done the dynamic mechanical load experiment by the applications ofthe mechanical load type bioreactor which can be monitoring on-line. Combinedwith the MTT colorimetric method, the comparative analysis of loading conditioneffects on cell proliferation was researched. The loaded cells was donemorphological observation and analysis by using the atomic force microscope.