Mutational Analysis Of The PPP2R1A And PPP2R1B Genes In Chinese Patients With Ovarian Cancer

Purpose:To determine the mutation frequencies of the paralogous PPP2R1A andPPP2R1B genes in Chinese patients with primary and secondary ovarian cancer, andto provide theoretical basis for the diagnosis and therapy of patients with ovariancarcinoma.Methods: A total of251FFPE slides from patients with distinct subtypes of ovariancarcinoma were recruited, the genomic DNAs were extracted, then followed by PCRamplification, PCR products purification. Finally, the PCR products were analyzed bydirect sequencing.Results:1. For PPP2R1A, a heterozygous, somatic mutation (c.771G>T, p.W257C)was identified in1out of37patients (2.7%) with primary ovarian endometrioidcarcinoma. The mutant sample was that of a46-year-old female, who was alsodiagnosed with ectopic endometriosis in the benign ovary.2. No PPP2R1B mutationswere detected in the remaining250patients with ovarian carcinoma.Conclusions:1. Population difference might be one of the reasons for the lesscommon frequency of PPP2R1A mutations in ovarian cancer patients from Chinarelative to that from Europe and America.2. For PPP2R1B, no mutations weredetected in our samples. Furthermore, our study also supported previous observationsthat PPP2R1B mutations were absent in ovarian cancer, suggesting that PPP2R1Bmutations are not actively involved in the pathogenesis of ovarian carcinoma.3. Ourresults showed that the PPP2R1A and PPP2R1B mutations might be not activelyinvolved in the pathogenesis of ovarian cancer in China; however, this speculationshould be further confirmed with larger sample size.