The Effect Of Golgi Fragmentation To Tau Hyperphosphorylation

Background: Alzheimer disease (AD) is an age-related neurodegenerative disorder,characterized pathologically with intracellular neurofibrillary tangles (NFTs),which mainly consists of hyperposphorylated microtube-associated proteintau. Because tau hyperphosphorylation is an important hallmark of the AD neuronsdegenerative, the study of tau hyperphosphorylation upstream factors in AD neuronaldamage mechanism is of great significance for effective prevention and treatmenttargets.Methods: C57BL/6mice were provided by the Experimental Animal Center of TongjiMedical College of Huazhong University of Science and Technology. Animals reared ina relaxed and suitable environment, free drinking water, room temperature wasmaintained at a constant temperature (26±2°C). All animal experiments werecomplied with animal use policies promulgated by the Society for Neuroscience.APP/PS1transgenic mouse brain tissue samples were got from Professor Zhang Yan ofPeking University. Made ultrastructural study of neurons for4,8,13and16-month-oldC57BL/6male mice and6months APP/PS1transgenic mice (n=4-5for each agegroup). Golgi matrix protein and tau protein expression were detected by Western Blotmethod in the4,8,13and16-month-old male C57BL/6mice (n=4-5for each age group) brain. Took analysis of the relationship between the matrix protein and tauhyperphosphorylation in immunofluorescence methods.Results: There were an age-dependent increasing of neuronal Golgi fragmentation andtau phosphorylation in the brain of C57BL/6mice and BFA and nocodazole couldinduce Golgi fragmentation with tau hyperphosphorylation in HEK293/tau cells.Conclusions: In AD mice, Golgi fragmentation increases with age increasing in neuroncells, and Golgi fragmentation is one of the upstream factors to tauhyperphosphorylation..