Study On The Clinical Significance Of High Expression Of SATB1in Esophageal Squamous Cell Carcinoma And Its Role In Promoting Metastasis

Backgrounds and aimsEsophageal squamous cell carcinoma is the most important Pathological type ofesophageal cancer(90%)[1].It is a aggressive and poor prognosis tumor. theaverage annual mortality rate of esophageal cancer in China is14.59/10million, and32.22/10million in Henan province.The major cause of death of the esophagealcancer patient is tumor invasion and metastasis. The study found that the specialAT-rich domain binding protein SATB1promote tumor invasion and metastasis byregulating chromatin structure and transcription sequence [2,3]. However,the rolesof SATB1on esophageal squamous cell carcinoma remains unknown.MethodsThe level of SATB1expression was analyzed in75pairs of esophageal squamouscell carcinoma tissue and esophageal inflammatory tissue. Western blot andReal-time quantitative RT-PCR to assess the SATB1expression in two differentinvasive esophageal cancer cell lines. Meanwhile, siRNA was constructed andsilienced the SATB1expression in human esophageal cancer cell lines Eca109and TE-13to evaluate the effects tumor invasion of SATB1.ResultThe expression level of SATB1in esophageal squamous cell carcinoma wassignificantly higher than inflammatory tissue (46/75); The tumor which expressedhiger SATB1level was significantly associated with clinical and pathologicalfeatures such as the degree of tumor differentiation, tumor infiltration depth,especially lymph node metastasis and distant metastasis;The expression of SATB1inTE-13cell line Significantly higher than in Eca109cell line(p<0.01).Compared withthe control group, siRNA Silence SATB1expression may down-regulating MMP2、MMP9and finally inhibit tumor cell invasion.Conclusion:SATB1could significantly raise the ability of cell migration and invasion inesophageal squamous cell carcinoma by up-regulating Matrix metalloproteinase2,9...