Establishment Of Chronic Rejection Animal Model By Heterotopic Tracheal Transplantation On Rat And The Role Of IL-17in The Model

OBJECTIVE：To establish a new heterotopic tracheal transplantmodel of rat.METHODS：Weight matched inbred rats of different germ lineswere randomly divided into2groups(n=20pairs／group): A,Wistar(donor)�'SD(recipient), B: SD(donor)�'SD(recipient). Theheterotopic tracheal transplant model was established by implanting thetrachea into anterior cervical tissues and muscles for blood supply.Tracheal graft rejection was monitored by clinical airway assessment,H-E staining for histological examination and observing the epithelialoss, lymphocytic infiltration and luminal occlusion of allografthistological.RESULTS: All the rats in two groups were alive. All the transplantsin group A had a certain degree of rejection reactions, while the group Bhad no special changes. By H-E staining, at the7th,14th,28th days, inthe2groups, the numbers of lymphocyte were:﹙9.40±1.71﹚HPF,﹙28.80±1.54﹚HPF,（31.50±1.95）／HPF and＜8HPF; the ratios of anuclear chondrocyte/osteocyte were〔（47.53％±1.38％） vs （47.93％±1.03％）,(p＞0.05),〔（81.83％±1.52％） vs（47.60％±1.04％）,(p＜0.05)〕 and〔(86.98％±1.71％) vs（47.14％±1.09％）,(p＜0.05)〕; ingroup A, epithelia loss were45%、100%、100%）,while in the group B,epithelia loss were not obviously. The incidence of OB in group A wassignificantly higher than the group B (p=0.000).At the7t h,14t h,28t h,inthe2groups, the expression of IL-17were 〔The experimental group:﹙10.40±2.31）﹙20.99±5.11）﹙91.64±11.49）,The control group:﹙10.05±1.67）﹙21.10±2.87）﹙17.60±2.08）〕CONCLUSION: Successfully establish the chronic rejection animal model byheterotopic tracheal transplantation on rat.And IL-17is relative to the developmentof the chronic rejection...