The Research Of The Migration Regulation Of Time And Space Of Newborn Neuron And Mechanism After Ischemia-reperfusion

Objective:Reelin is the migration signal of new neurons signal in brain development. Reelin mutant mice showed reeler phenotype, this kind of reeler mice are the characteristics of the cerebral cortex lack of layered structure, in the adult brain, Reelin continuously express, experiments have shown that in cerebral ischemia injury, the number of new neuron in Reelin gene mutations reeler adult mice decrease sharply in the dentate gyrus and beak latera migration flow This shows that not only in neuron development, but also in the adult brain. reelin still have influence on neuron migration. This paper mainly studies the brain ischemia reperfusion injury, the regulation of the area of SVZ of new neurons in the time and space.Methods:MCAO rats (middle cerebral artery embolization) reperfusion model, respectively in cerebral ischemia reperfusion after60minutes24h,72h,7d after materials, using fluorescent immunohistochemistry method observation SVZ (subependymal region) differentiation and migration of neuron expression in the above time point change. giving medicine RAP (block reelin-dabl signal path) to lateral ventricle, the measure of ischemic area dabl express quantity by western blotting Fluorescent double scale immunohistochemical evaluation whether the migration neurons and dab common location. With fluorescent immunohistochemistry method detection after the treatment SVZ area differentiation and migration of neurons express.Results:1. Western blotting results:and control group ratio, cerebral ischemia reperfusion injury at the same time give medicine RAP1d,3d,7d,, phosphorylation dabl expression a quantity to have significantly reduced (<0.05>.2. Immunof luorescence results:(1) the control group,1d,3d,7d, are seen in the differentiation of neurons (ki67mark) positive cells. And the control group, compared to cerebral ischemia reperfusion injury in the differentiation of neurons (ki67mark) increased significantly (p<0.05), ischemia-reperfusion1days most positive cells after ischemia,3d,7d positive cells in order to reduce the number.(2) in the control group,1d,3d,7d are seen in the migration neurons (PSA-NCAM) positive cells, and the control group, compared to ischemia-reperfusion injury after7d neurons significant increase no statistical significance (p>0.05). After ischemia1d,3d, in the migration neuron number increased significantly (p<0.05).(3) ischemia side and not ischemia side compared with area differentiation of neurons increased significantly (p<0.05).(4) to medicine RAP and compared, in the migration neuron number of positive cells was significantly reduced (p<0.05). And the differentiation of neurons express quantity was not statistically significant.3. Immunof luorescence double scale results:we adopt phosphorylation dabl (dabl is reelin downstream signal molecules, phosphorylation will start after the downstream signal path), and migration, neuron left fluorescence double mark, found that phosphorylation dabl and migration, neuron positive cells were positioning.Conclusion:1. Rats after reperfusion injury, differentiation and migration of neurons were significantly increased, and ischemic area and not ischemic area compared with the differentiation of neurons increased significantly.2. Give medicine after RAP phosphorylation dabl expression significantly reduce, thus RAP blocked Reelin-dabl signal path. And RAP resistance segment Reelin-dabl signal path later, and the control group, compared to the neuron migration significantly reduced, and the differentiation of neurons express quantity did not change. Show that Reelin-dabl signal path mediated migration of new neurons.