Study On EGFR, VEGF And PTEN In The Esophageal Carcinoma Tissues

Objective: To investigate the protein expression of epidermal growth factorreceptor (EGFR), vascular endothelial growth factor (VEGF) and phoshatase andtensin homology deleted on chromosome ten (PTEN) in esophageal carcinoma (EC)and their relationships with clinicopathological parameters of EC.Methods:Sixty postoperative patients with EC in Anyang Tumor Hospital werecollected, and60cases of corresponding paraneoplastic normal tissues were collectedas a control. The protein expressions of EGFR, VEGF and PTEN in the EC tissues andthe corresponding paraneoplastic normal tissues were detected by usingimmunohistochemistry MaxVision method. The protein expressions of EGFR, VEGFand PTEN in the EC tissues and the corresponding paraneoplastic normal tissues wereanalyzed comparatively by using SPSS17.0; and their relationships withclinicopathological parameters of EC were analyzed too; and the correlations of threeindexes were analyzed by using Spearman method.Results:The protein expression positive rates of EGFR, VEGF and PTEN in theEC tissues were56.67%,66.67%and46.67%, and those in the paraneoplastic normaltissues were11.37%,10.00%and96.67%(P<0.05).The protein expression positiverates of EGFR in the EC tissues with phase I and II (29.41%) was lower than that inthe EC tissues with phase III and IV (67.44%)(P<0.05). The protein expressionpositive rates of EGFR in the high differentiated, medium differentiated, poorlydifferentiated EC tissues were33.33%,65.38%,94.74%, respectively (P<0.05). Theprotein expression positive rates of EGFR in the EC tissues with lymph nodemetastasis (78.26%) was higher than that in the EC tissues without lymph nodemetastasis (43.24%)(P<0.05). The protein expression positive rates of EGFR in theEC tissues with extra-esophageal infiltration (73.68%) was higher than that in the ECtissues without extra-esophageal infiltration (27.27%)(P<0.05). The protein expression positive rates of VEGF in the EC tissues with phase I and II (47.06%) waslower than that in the EC tissues with phase III and IV (74.42%)(P<0.05). The proteinexpression positive rates of VEGF in the high differentiated, medium differentiated,poorly differentiated EC tissues were13.33%,57.69%,89.47%, respectively (P<0.05).The protein expression positive rates of VEGF in the EC tissues with lymph nodemetastasis (86.96%) was higher than that in the EC tissues without lymph nodemetastasis (54.05%)(P<0.05). The protein expression positive rates of VEGF in theEC tissues with extra-esophageal infiltration (86.84%) was higher than that in the ECtissues without extra-esophageal infiltration (31.82%)(P<0.05). The proteinexpression positive rates of PTEN in the EC tissues with phase I and II (76.47%) washigher than that in the EC tissues with phase III and IV (44.19%)(P<0.05). Theprotein expression positive rates of PTEN in the high differentiated, mediumdifferentiated, poorly differentiated EC tissues were93.33%,53.85%,21.05%,respectively (P<0.05). The protein expression positive rates of PTEN in the EC tissueswith lymph node metastasis (26.09%) was lower than that in the EC tissues withoutlymph node metastasis (70.27%)(P<0.05). The protein expression positive rates ofPTEN in the EC tissues with extra-esophageal infiltration (39.47%) was lower thanthat in the EC tissues without extra-esophageal infiltration (77.27%)(P<0.05). Theprotein expression ofEGFR,VEGF and PTEN was not related with the gender, age andpathological shape (P>0.05).The expression of EGFR was positively correlated withthat of VEGF (r=0.452, P<0.05); the expression of EGFR and VEGF was negativelycorrelated with that of PTEN in the EC tissues (r=-0.616,-0.520; P<0.05).Conclusion:The EGFR and VEGF proteins of EC tissues are highly expressed,and the PTEN protein is lowly expressed. The EGFR, VEGF and PTEN proteinexpressions of EC tissues are related with the clinical stage, degree of differentiation,lymph node metastasis and depth of tumor infiltration; and have no correlation withgender, age and pathological shape. The expression of EGFR was positively correlatedwith that of VEGF; the expression of EGFR and VEGF was negatively correlated withthat of PTEN in the EC tissues. The high expressions of EGFR and VEGF protein andlow expression of PTEN protein may be involved in the development and progressionof EC, they can be used for the diagnosis and prognosis of EC, and maybe become thenew target points of EC treatment.