Mechanism And Experiment Investigation About The Agglomeration State Of Immune Complex Oriented To Variable Magnetic Separation

Chemiluminescent immunoassay used for detection and prevention of theserious diseases is the most advanced immunoassay technique at present. Theimmune complex by the specific reaction coated with some special markers, such asantigen-antibody-antigen, can produce photons under the action of oxidant orcatalyst. The concentration of the target can be got by the relation with the quantityof photons. It’s necessary to separate the immune complex and the uncombinedmarkers, mainly adopting the permanent magnetic field. However, the immunecomplex can cluster under the permanent magnetic field and the uncombined freephase can be buried in the immune complex easily. The quantity of photonsproduced by the uncombined and residual markers will affect the detection results ofthe photons, which is likely to lead to the false positive results. So the research aboutthe agglomeration state of the immune complex has significance to the improvementof the precision of chemiluminescent immunoassay.Firstly this paper studies the time of full absorption needed by the magneticbeads under the action of the magnetic field and flow field. The force acting on theindividual particle is analyzed in suspension liquid of magnetic beads, and thedifferential equation about the magnetic bead’s movement is got. Using MATLABsoftware to solve the equation, the displacement curve is obtained. It lays atheoretical foundation for the further study, and it can guide the action time of thechanging magnetic field.Secondly, based on the theory of magnetic dipole, the paper studies theagglomeration state of the magnetic beads under the action of the magnetic field, andanalyzes the motion of immune complex under the action of the vibration of themagnetic field. The conclusion is that the vibration of the magnetic field can makethe magnetic particles be in the status of vibration, and can achieve the objective ofeliminating the phenomena of cluster. At last, the conditions that the beads can’tgather into cluster are analyzed, and the principle of the selection of vibrationparameters is put forward.Finally, this paper builds an inverted and microscopic observation system,which can realize the visualization of immune complex’s response in the magnetic field, and implements the experimental study on the impact of the vibration field onthe motion state of the magnetic beads. On the basis of the existing set of magneticseparation device, using the converse piezoelectric effect, the piezoelectric ceramicis used to drive the device to vibrate in a tiny displacement to form a variablevibration field. Its frequency and amplitude is easy to adjust. Due to the combinationwith the microscope, the process of mechanism design should take the size of themicroscope into account.The observation experiments on the morphology and adsorption of magneticparticles are conducted, as well as the observation experiment on the vibratory stateof magnetic beads. The study on the previous motion model and the vibration stateof the magnetic beads is verified. At the same time, the experimental images fromthe observation system can be transmitted to the computer to be stored and analyzed.The agglomeration state of the immune complex is studied, and the feasibilitythat the variable magnetic field can be used to eliminate the phenomenon ofclustering is verified. At the same time, the suitable parameters of the variablevibration field is explored. The research has certain reference significance for thedesign of the variable magnetic separation system.