The Effects Of CSF-1Antisense Oligodeoxynucleotide To The Expression Of CSF-1Gene And Cell Proliferation On The Ovarian Carcinoma SKOV3Cell Lines

Objective: To investigate the CSF-1antisense oligodeoxynucleotidestransfixion of ovarian cancer SKOV3cells interfere with the gene expressionand inhibition of cell proliferation. Methods: the synthesis of CSF-1antisense oligonucleotides, with X-tremeGENE siRNA Transfixion Reagent wastransfixed into SKOV3cells in ovarian cancer. The experiment was divided intofour groups: the experimental group that antisense group (SKOV3cellstransfixion reagent+antisense oligonucleotides), missense group (SKOV3cellstransfixion reagent+missense oligonucleotides), negative control group(SKOV3cells transfixion reagent),control group (SKOV3cells do not do anyprocessing). Using RT-PCR and Western blotting were assayed by CSF-1aftertreatment of mRNA and protein expression levels, through was detected byMTT cells after treatment with cell proliferation inhibition. Results: theapplication of CSF-1antisense oligodeoxynucleotides transfixion of SKOV3cells in ovarian cancer, the experimental group CSF-1mRNA and proteinexpression level significantly decreased, cell proliferation was inhibited,compare with the other three groups, the difference was statistically significant(P<0.01). The experimental group gene silencing efficiency is62.8%, protein 55.6%inhibition, inhibition of cell proliferation rate of57.4%. Conclusions:targeting CSF-1antisense oligonucleotides could inhibit the growth of ovariancancer cell SKOV3CSF-1expression, to a certain extent, the inhibition ofSKOV3cell in vitro proliferation, induce apoptosis, so CSF-1antisenseoligonucleotides for gene therapy of ovarian cancer has certain potential inmeaning.