Study On The Phenolic Compounds Isolated From Jasminum Officinale L. Var Grandiflorum And Their Bioactivities For Anti-HBV

Hepatitis B virus (HBV) infection can cause acute or chronic hepatitis B,which may lead to cirrhosis and hepatocellular carcinoma (HCC). Up to date,there are still no specific drugs to treat hepatitis B due to the continuous cycleof the replication of Hepatitis B virus, mutant strains of the virus and thebody’s immune clearance reaction. China has the treasure house of Chineseherbal medicine. Screening anti-HBV natural compounds with highbioactivity，low toxicity，new structures from Traditional Chinese Medicine isimportant to perform anti-HBV natural active pharmaceutical research.Jasminum officinale L．var．grandiflorum MuXi secco jasmine plant forJ.officinale dry flower buds, alias element, and zilpah mingyuan warm-heartedneedle flowers. It was cultured around the world due to the medicalapplication with the effect of Shu-Gan-Jie-Yu and gas painkillers．In ourcountryside, it is used to treat maldigestion, duodenal ulcer, chronic hepatitisbulbar urethra, cirrhosis and other disorders. Therefore, based on the previousliteratures, we chose Jasminum officinale L.var. grandiflorum as the rawmaterial due to its good efficacy of treating liver disease. The differentchemicals will be isolated from it and the anti-HBV activity will be evaluatedin HepG2.2.15cells to screen the specific chemicals for anti-HBV.After using chloroform and n-butanol to extract70%ethanol extract fromthe flower buds of J．officinale, six phenol monomer compounds were isolatedand purified. The structures of these compounds were confirmed by1H-NMR and13C-NMR. They are scopoletin(1), ethylgallate(2),1,4-benzenediol(3),2-(4-hydroxybenzyl)-malic acid(4), salidroside(5) and p-Hydroxy phenylet--hanol(6). Compounds1~5were isolated from Jasminum officinale at thefirst time.Then the anti–HBV activity of ethylgallate was evaluated by thepreliminary Anti–HBV activitiy assay using HepG2.2.15cell line, whichdisplayed the certain inhibition of the secretion of HBsAg with62.5μg/ml ofIC50.In summary, we isolated5new active fractions of phenol monomercompounds from J. officinale dry flower buds and found one of thesecompounds has the anti-HBV activity (ethylgallate). It proved that the phenolcompound also has bioactivity besides the well-known triterpenoid saponinsand iridoid compounds from J.officinale, which will be helpful to extend theresearch for the Traditional Chinese Medicine of J. officinale and to providethe scientific data for the new formulations. Moreover, it will find the basis ofanti-HBV activity of J. officinale and the relationship between the structureand activity by means of parallel investigation of phenolic compounds,triterpenoid saponins and iridoid compounds from J.officinale. Furthermore, itwill broad the scope of anti-HBV drugs from J.officinale throughderivatization. Finally, the medicinal value for anti-HBV of J.officinale will beenhanced by building the optimized extraction and separation technology andquality standards, even evaluation of anti-HBV activity in vivo.