Hplc Fingerprint Chromatogram Of The Root Of Mallotus Apelta And Its Chemical Composition

This article dealed with the study on the root of Mallotus apelta, including the HPLC fingerprint, chemical composition research and quantitative analysis studies based on literature review. The aims are to establish quality standard and control the quality of the root of Mallotus apelta medical materials effectively in all aspects.The fingerprint of the root of Mallotus apelta was studied by using HPLC technology and at the same time, matched and assessed similarity evaluation of the relevant parameters of the chromatograms about the root of Mallotus apelta by a software "similarity evaluation system for chromatographic fingerprint of TCM"(version2004).10batches of materials come from different origin.15common peaks were demarcated and4peaks of them respectively were identified. The similarity value was above0.9, which indicated no difference about chemical component of samples from the10different areas.Four compounds were separated from the parts of ethyl and n-butyl alcohol of the root of Mallotus apelta by Semi-preparative high performance liquid chromatography. Their structures were gallic acid,1-O-galloyl-6-O-luteoyl-α-glucose,3’-O-methyl-ellagic acid-4-O-α-L-rhamnopyranoside and5’-demethyl aquillochin, respectively. The4compounds were isolated from this medicinal materials for the first time. All the structures were identified in the help of13C-NMR、1H-NMR and literature data. The advantages of the Semi-preparative high performance liquid chromatography method were simple and fast.The content of3’-O-methyl-ellagic acid-4-O-α-L-rhamnopyranoside was measured by HPLC at the wavelength of254nm and the retention time of3’-O-methyl-ellagic acid-4-O-α-L-rhamnopyranoside at about9min. The content of3’-O-methyl-ellagic acid-4-O-α-L-rhamnopyranoside was between0.47to1.67mg/g about10batches of the root of Mallotus apelta, which were considerable differences. The HPLC method for determination the content of3’-O-methyl-ellagic acid-4-O-α-L-rhamnopyranoside had high sensitivity and good reproducibility.The study provided the scientific basis for identifying positive or negative herbs, and, controlling the quality of the root of Mallotus apelta.