A Study On Rhoe-induced Apoptosis In Human Glioma Cells

Objective:To evaluate the roles of RhoE in the course of apoptosis of human glioma cells.Methods:Expression of RhoE in glioma cell lines(human polymorphism malignantglioma-T98、human glioblastoma-A172、human brain stellate mother cell-U87) wasdetected by western blotting; pcDNA3.1-RhoE was generated and transientlytransfected into U87cell line, methyl thiazolyl tetrazolium (MTT) assay wasperformed to determine the effect on proliferation; Hoechst staining and Flowcytometric analysis were used to detect the effect on apoptosis; western blottingfurther assessed the change on protein expression of Bcl-2and Bax, as well as activityof caspase-3and NF-kB.Results:1、Compared with normal human astrocytes, glioma cell lines such as T98, A172and U87cells all show low protein expression of RhoE(P<0.05).2、up-regulating RhoE obviously inhibited proliferation of U87cells and inducedcellular apoptosis(P<0.05).3、up-regulating RhoE, western blotting also showed increase of cleave caspase-3and Bax protein level, but decrease of Bcl-2.4、up-regulating RhoE, NF-κB subunit p65nucleus show low protein expression.Conclusions:RhoE molecules could be playing an important role in the occurrence anddevelopment of glioma, up-expressing RhoE could induce apoptosis of glioma U87cells, which may involve decreased activity of NF-κB and ratio of Bcl-2/Bax, andsubsequently increased cleaved caspase-3. RhoE molecules may be a potentialtumor-suppressor genes in the occurrence and development of glioma.