Extraction And Determination Of The Luteolin From The Chrysanthemum

The chemical constituents of Chrysanthemum were separated by Silica gel columnchromatography. The chemical structures of the compounds were determined by¹HNMR,¹³CNMR and DEPT spectra etc，one compound was isolated and identifieded as Luteolin.Luteolin,3’,4’,5,7-tetrahydroxyflavone is an important member of the flavonoid family, whichdemonstrates a wide range of biochemical and pharmacological effects, includinganti-oxidation,anti-bacteria, anti-virus, anti-inflammatory, anti-carcinogenic and otherbeneficial properties.Graphene is a carbon material closely packed into a single layer of carbon atoms, hascaused widespresd concern in the field of chemistry, great power is showed in the fields ofcatalysis、sensors. The conditions of preparation of graphene is contrasted and optimized inthe experiment，optimal conditions is found in preparation of grapheme, graphene solutionstable at room temperature is prepared.A simple and highly sensitive electrochemical method was developed for thedetermination of trace-level luteolin, based on the copper（Cu） nanocluster/grapheme（GR）-modified electrode. Scanning electron microscopy（SEM）, Energy DispersiveSpectrometer（EDS） were used for the characterization of the distribution of the Cunanoclusters on the grapheme matrix. The composite of the Cu–GR was investigated by theelectrochemical characterization of cyclic voltammetry （CV） and electrochemical impedancespectroscopy （EIS）. The preliminary study shows that the sensor has synergisticelectrocatalytic activity to the oxidation of luteolin. The linear range for the detection of theluteolin is7.0×10^-7to3×10^-6mo·L^-1, a low detection limit of3×10^-8mo·L^-1, the standardsolution and actual sample was measured in the paper, the result that the chemically modifiedelectrode has good reproducibility and accuracy are demonstrated. in this experiment theinfluences of glucose,citric acid,cyclodextrin, were investigated, the results show that thiselectrode has good effect in determination of luteolin, has advantages of stronganti-interference ability, applicable to the determination of luteolin.