The Research Of The Correlation Between The Transcription Factor ETS-1and B Cell Abnormality In The Pathogenesis Of Systemic Lupus Erythemaotsus

Objective To investigate the influence of transcription factor ETS-1mRNA and Blymphocyte-associated cytokines on the differentiation of B cells in SLE patients, andexplore its significance in the pathogenesis and clinical features of SLE.Methods Thirty-one SLE patients (29females and2males, age range16～55years,mean age33.77±11.21years,) who ful-filled at least four of the revised SLE criteriaof the American College of Rheumatology were all randomly selected from theDepartment of Rheumatology&Immunology of Anhui Provincial Hospital. Therewere no autoimmune disease, tumor and infection was excluded. Twenty of thepatients were active SLE patients (SLEDAI score>5), the remaining11SLE patientswere in remission (SLEDAI score≤5). Fifteen healthy volunteers served as a healthycontrol population, include14females and1males, age range23-32years, mean age30.33±3.87years, ex-and age-matched.The peripheral blood mononuclear cell（PBMC）were purified on Ficoll densitygradient. PBMC layer was collected, flow cytometry was used to detect the change inthe ratio of CD19-CD138+plasma cells and CD19+B cells. and CD19+B cellswere isolated using magnetic bead separation technology. The different levels ofETS-1mRNA in B cells were determined by real-time PCR. Collected of SLEpatients and normal serum stored at-80℃. IL-10、APRIL in supernatants wasdetected with ELISA. The correlation of levels of Ets-1mRNA of B cells in thesesubsets, the ratio of CD19-CD138+/CD19+and the levels of IL-10and APRIL inserum with the clinic and laboratory parameters of SLE were analyzed. Result①Compared to the healthy controls, SLE patients showed decreased mRNAexpression level of ETS-1in B cell（sZ=-4.218，P<0.01）.②The percentage of CD19+B cells was decreased, but CD19-CD138+plasma cells and CD19-CD138+B cells/CD19+plasma cells ratio were increased in patients with SLE compared with healthycontrols(p<0.05).③T he expression level of ETS-1mRNA was significantly negativecorrelated with the proportion of CD19-CD138+plasma cells （r=-0.359，P=0.047）,negative correlated with the CD19-CD138+B cells/CD19+plasma cellsratio （r=-0.493,P=0.005）.However, there is no correlation in normal controls.④Thelevels of IL-10and APRIL in active SLE patients were significantly higher than thatin control, and shows no significant correlation with the CD19+B cells ratio,significant correlation with CD19-CD138+plasma cells and the ratio ofCD19-CD138+B cells/CD19+plasma cells.⑤It shows significant negativecorrelation between the expression level of ETS-1mRNA in B cells and the level ofIL-10and APRIL in serum of active SLE patients, but no correlation was found ininactive group.⑥SLE patients showed negative correlation between the expressionlevel of ETS-1mRNA and titer of ANA（r=-0.376,P=0.041）.Conclusion Ets-1may participate in the pathogenesis of SLE by impact on thedifferentiation of B cells and cooperation with B cell differentiation-correlatedcytokines, such as IL-10、APRIL.