The Association Between Paraoxonase1Gene Polymorphisms, Oxidative Stress And Retinopathy In Type2Diabetes Mellitus

Objective To investigate paraoxionase gene polymorphisms (Q192R and L55M) andoxidative stress in type2diabetes mellitus retinopathy of Han people in Anhuiprovince. To analyse the relationship between PON1gene polymorphism, oxidativestress and type2diabetic retinopathy.Methods (1) We conducted a case-control study on a total of184patients with type2diabetes, comparing those with retinopathy (n=94) and without retinopathy (n=90).(2)Paraoxionase1gene polymorphisms (Q192R and L55M) were determined by PCR-RFLP in136normal control subjects and184type2diabetes patients with variousdegree of retinopathy.(3) Use spectrophotometer to detect serum MDA content andSOD activity in those people, in order to understand the body oxidative stress.Results (1) As compared with NDR patients, DR patients had higher systolic bloodpressure, LDL, glycated hemoglobin and longer duration. BMI, diastolic bloodpressure, triglyceride, cholesterol and HDL did not differ significantly between twogroups. As compared with NPDR patients, PDR patients had higher diastolic pressureand longer duration.(2)The QQ genotype was significantly more frequent in DRpatients compared with NDR patients (P<0.05). There was no difference in QQgenotype between T2DM and NGT, or between PDR and NPDR (P>0.05).(3) Therewas no difference in PON155L/M gene in this study.(4) DR patients had lower SOD activity and higher MDA content than NGT group and NDR group. PDR patients hadlower SOD activity and higher MDA than NPDR group.(5) In NDR and DR group,192QQ genotypes had lower SOD activity and higher MDA content. There was nodifference in NGT group.Conclusion (1) With longer duration of diabetes, incidence of retinopathy increased.Achieving blood glucose, blood pressure, lipid control may delay the occurrence anddevelopment of DR.(2) PON1Q192R was significantly associated with retinopathy intype2diabetes，but it was not associated with the progression of DR, Q allele may be agenetic risk factor for DR.(3) PON1L55M was not associated with DR.(4) Oxidativestress may play an important role in the occurrence and development of diabetes anddiabetic retinopathy. Lower oxidative stress may be a protective effect for DR.(5) InNDR and DR patients, PON1gene polymorphisms may affect oxidative stress.