Polymorphism Of ERAP1Gene With Yunnan Non-small Cell Lung Cancer Occurrence And Development Association

[Objective]To study ERAP1gene mutation and the occurrence and development of non-small cell lung cancer; the ERAP1gene results obtained to construct the ERAP1gene mutation database.[Method]Using real-time quantitative polymerase chain reaction (RT-PCR), TaqMan fluorescence probe technique and sequencing method,131cases of Yunnan nationalit y in non-small cell lung cancer (NSCLC) ERAP1gene rs26653were patients and179healthy controls from Yunnan; rs27044; rs30187; four SNPs rs26618gene typing, in between the two groups in the frequency calculate all alleles and genotypes of haploty pe, haplotype frequency calculation. Yunnan were calculated for each genotype and th e risk of non-small cell lung cancer, to explore the relationship between clinical param eters of each genotype and non small cell lung cancer.[Results]1. rs27044in the case group and the control group in the C. G allele frequencies were40.5%,59.5% and47.5%,52.5%./=3.23. P=0.08. rs27044alleles C G frequency in Ⅰ+Ⅱ and Ⅲ+Ⅳ non-small cell lung cancer patients was50%,50% and36%,64%, there were significant differences (x2=4.673, P=0.031). CC, CG and GG three genotypes were genotype frequency non small cell in patients with Ⅰ+Ⅱ and Ⅲ+Ⅳ in26%,48%,26%and10%,52%,38%, there were significant differences in genotype frequencies (X2=6.124, P=0.047)2. rs26653in case group and control group in C, G allele frequencies were55%,45%and44.7%,55.3%,(X2=6.38, P=0.01). rs26653alleles C, G frequency in Ⅰ+Ⅱ and Ⅲ+Ⅳ non-small cell lung cancer patients was46%,54%and59%,41%, there were significant differences (x2=3.637, P=0.057). CC, CG and GG three genotypes were genotype frequency non small cell in patients with Ⅰ+Ⅱ and Ⅲ+Ⅳ in26%,41%,33%and30%,57%,13%, there were significant differences in genotype frequencies (x2=8.303, P=0.016).3. rs26618loci in the case group and the control group in the A, G allele frequencies were64.9%,35.1%and75.4%,26.4%(x2=8.15, P=0.004). The rs26618allele frequency in A, G,Ⅰ+Ⅱ and Ⅲ+Ⅳ non-small cell lung cancer patients was64%,36%and65%,35%, no significant difference (x2=0.02,P=0.89). AA, AG and GG three genotypes were genotype frequency non small cell in patients with Ⅰ+Ⅱ and Ⅲ+Ⅳ in43%,43%,14%and42%,47%,11%, there were significant differences in genotype frequencies (X2=0.346, P=0.84).4. rs30187loci in the case group and the control group in the A, G allele frequencies were42.4%,57.6%and50.3%,49.7%(x2=3.80, P=0.051). The rs30187allele frequency in A, G in Ⅰ+Ⅱ and Ⅲ+Ⅳ non-small cell lung cancer patients was50%,50%and39%,61%, no significant difference (x2=2.951, P=0.086). AA, AG and GG three genotypes were genotype frequency non small cell in patients with Ⅰ+Ⅱ and Ⅲ+Ⅳ in29%,43%,29%and11%,55%,34%, there were significant differences in genotype frequencies (x2=6.171, P=0.046).5. construction of haplotype rs26653/rs27044/rs30187/rs26618. According to the lin kage disequilibrium:the study of lung cancer group and the control group with CGGA (OR:0.929.95%CI:0.623～1.387; P=0.719), CGGG (OR:0.625,95%CI:0.439～0.890; P=0.008), GCAA (OR:1.312,95%CI:0.948-1.815; P=0.101), GGAA (OR:1.260,95%CI:0.491-3.237; P=0.629), GGGA(OR:2.720,95%CI:1.052-7.031; P=0.032) fo ur kinds of haplotypes.[Conclusion]1. ERAP1gene polymorphism in the Yunnan area of non small cell lung cancer related.. rs26653, rs26618alleles and Yunnan non small cell lung cancer. rs27044genotypes and non-small cell lung cancer patients is related to the development of.2. Susceptibility of4haplotypes of ERAP1gene rs26653/rs27044/rs30187/rs26618-CGGG haploid type of non small cell lung cancer may have a protective role. Risk factors of4haplotypes of ERAP1gene rs26653/rs27044/rs30187/rs26618-GGGA haplotype may. for non-small cell lung cancer. The ERAP1gene of haplotype rs26653/rs27044/rs30187/rs26618-CGGA single haplotype may progression of lung cancer risk factors.