| Inhalation injury is respiratory and lung damage which caused by the heat and smoke, and it’s still the leading cause of death in the early stage of burn patients. Because it’s pathogenesis of inflammatory mediators and peroxidation closely related, and it can cause systemic inflammatory reaction, so the clinical treatment is still a difficult issue. Edaravone is a novel potent antioxidant, it has an important role in anti-inflammatory factor and free radical scavenging. For this reason, through the application of edaravone intervene in the pathophysiological process of inhalation injury, it can be expected to protect the lung function.Objectives:1.Detect concentration levels of interleukin-6(IL-6), interleukin-10(IL-10),tumor necrosis factor-a(TNF-a) in serum, to research the effects of edaravone on inflammatory mediators in the early stage of smoke inhalation injury.2.Detection activity of malondialdehyde(MDA), myeloperoxidase(MPO) and superoxide dismutase(SOD) in lung homogenates, to research the effects of the edaravone on oxidation and anti-oxidation factorin the early stage of smoke inhalation injury.3.Through this experiment, we can observe the effect of edaravone on inflammatory mediators and oxidant/antioxidant balance in rats with smoke inhalation injury, investigate whether edaravone has a protective effect on early smoke inhalation injury, and its mechanism of protective effect.Methods:Thirty-two male SD rats were divided into four groups randomly (n=8):normal control group (A), injured group(B), edaravone prevented group(C), edaravone treated group(D).Ten minutes before smoke inhaled injured, the rats in group C were all injected intraperitoneally with edaravone (9mg/kg); thirty minutes after smoke inhaled injured, the rats in group D were all injected intraperitoneally with edaravone (9mg/kg), and in the meantime the group B were injected the same volume normal saline.The rats were with no dispose in control group. Every group collected the blood samples5ml from femoral artery and the lung tissue after the injured at the time of6hours. Centrifugalized the samples and detect the levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-10(IL-10) in serum as well as the levels of malondialdehyde(MDA), myeloperoxidase(MPO) and Superoxide dismutase(SOD) in lung tissue. The histopathological examination was performed with the tissue specimens taken from the apex of right lung of the rats.Results:1.General observation with ordinary circumstances and specimen:Observation of the general:Because the rats were randomized to use the random number table, the weight of rats between the groups was not statistically significant. With no death in the injury process, the rats that had suffered from inhalation injury appeared dysphoric, had tachycardia and tachypnea. After move the rats to the ventilated place5-10minutes,these symptoms disappeared gradually. The rats in blank control group appeared to have stable respiration. General observation with the specimens which were taken after rats were killed:The lungs of rats of blank control group (group A) on visual inspection had normal color and no congestion and edema. The Wound blank group (group B) was found to have severe diffused and passive lung congestion. The edaravone treatment group(group C) was found to have ecchymosis and edema. The lung tissues of rats in the edaravone prevention group(group D) congestion significantly reduced,only with a small amount of bleeding points.2.Macroscopic observation:The lungs of rats from blank control group (group A) on visual inspection had normal color and no congestion and edema. and were found to have Little exudate. The alveolar septa of the Wound blank group (group B) was found to be obvious uneven, and have infiltrating inflammatory granulocytes.The lung tissues from the edaravone treatment group(group C) was found to have uniform alveolar septa, and have infiltrating inflammatory granulocytes. The lungs’ alveolar structure of the edaravone prevention group(group D) is clear, only have little infiltrating inflammatory granulocytes.3.The results of TNF-α、IL-6and IL-10:The edaravone treatment group(group C) was compared with the Wound blank group (group B), the level of IL-6and TNF-a were decreased (P<0.01), while IL-10was increased (P<0.01). And to the edaravone prevention group(group D),the above-mentioned is more obvious. 4.The results of MDA、MPO and SOD:The edaravone treatment group(group C) was compared with the Wound blank group (group B),the level of MPO and MDA were decreased (P<0.01), while SOD was increased (P<0.01).And to the edaravone prevention group(group D),the above-mentioned is more obvious.Conclusions:1. After the Inhalation injury, the lung tissue was congestion and edema, the alveolar structure is destroyed, the alveolar septa extremely uneven,and a large number of inflammatory cell infiltrating. The edaravone treatment group and the edaravone prevention group can make the injury be attenuated, the above-mentioned is more obvious in the prevention group.2. Edaravone has a protective effect on smoke inhalation injured by scavenging free radical and inhibiting proinflammatory cytokines.and prophylaxis is better. |
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