Establishment Of Mice Model Of Graves Disease

Objective:Intramuscular injection of recombinant plasmid pcDNA3.1/TSHR268with the technique of electroporation (EP) with female BALB/c mice was adopted to constract animal model of Graves diseases(GD).Methods:The plasmid pcDNA3.1/TSHR268was extracted by plasmid extraction kit and verified by restricting enzyme digestion and used as the material of gene immune. After mice were randomly divided into two groups,the BALB/c mice were immuned on weeks1、4、7with recombinant plasmids with EP. Blank group were injected with normal sodium.Mice were bled at weeks0,2,5,8at angular vein. The level of thyroxine(T4)、thyroid stimulating hormone receptor N-terminal antibody (TRAb N) and thyroid stimulating hormone receptor C-terminal antibody(TRAb C) were assayed. Histological analysis of thyroid tissue and99mTcO4-imaging were carried out on week9.Results:The method of gene immune combined with EP can successfully construct the model of GD.The level of T4increased from26.070±2.825ng/mL to60.321±14.423ng/mL (P＜0.05); The level of TRAb N increased from0.287±0.0372mIU/ml to0.538±0.154mIU/ml (P＜0.05); The level of TRAb C increased from12.277±2.952AU/ml to28.601±4.523AU/ml (P＜0.05). The higher thyroid uptake capacity of99mTcO4-was seen in experimental group than control group.The follicular epithelial cells hyperplasia and infiltrative inflammatory cells were observed in thyroid tissue. None difference of histological analysis were seen in control group. The sera T4、TRAb N and TRAb C of control group showed no statistically significance(P＞0.05).Conclusion:The method of recombinant plasmid pcDNA3.1/TSHR268combined with EP successfully constructed ideal animal model of GD.