Effect Of Gastrodin On In Vitro Culture Schwann Cells Proliferation And NGF Expression

Objective: To observe the effect of Gastrodin on Schwann cells proliferat-ion in vitro culture.Methods: the sciatic nerve was removed from the SD rats withmicroscope sterile, then cut into pieces and cultured in medium. S-100immunohistochemistry was used to identified the purity of the Schwanncells. The purified cells were cultured in DMEM medium with0.1mg/ml,0.05mg/ml，0.025mg/ml gastrodin serum. MTT assay was used to draw thecell growth curve, and the NFG expression within the Schwann cells wasmeasured with the confocal laser scanning microscope technology.Results:48h after primary culture, the observation showed that Schwanncells was adherent. Schwann cells have elongated or round nucleus, with renderthree-dimensional and plump fusiform body. The cells arranged side by side in5th days, and network structure or whirlpool sample arrangement can beobserved at10days after culture. The purity of the Schwann cells can reach92%by S-100immunohistochemistry identify.MTT assay results showed: in thenormal control group, the Schawann cells increased rapidly from24h to72h, butslowed down72h after culture. And Schwann cells become expanded and thethird dimension become weak at5th days. The growth trend in0.1mg/ml gastrodin was similar to the normal control, but the peak value at each time waslower than the control with obvious difference at48h. In the0.05gastondingroup, Schawann cells increased rapidly, and appeared difference compared with0.1mg/ml gastondin group at48h and compared with the control at72h. In the0.025mg/ml gastrodin group, the proliferation and differentiation of cells wassimilar to the normal. The Schwann cells in0.025mg/ml appeared differencecompared with the0.1mg/ml group at48h, and compared with0.05mg/ml at72h.Laser confocal scanning microscope(LCSM) auto collect image showed that theexpression level of NGF in Schwann cells in0.05mg/ml Gastrodin grouop washigher than any other groups, but lowest when Gastrodin concentration was0.1mg/ml.Conclusions:1. Schwann cells proliferation and differentiation can directlyaffect the expression of the NFG.2. Continuous application of0.1mg/ml Gastrodin sustain caninhibit Schwann cells proliferation and differentiation, as well as NGF express-ion. But the effect is very little when gastrodin density is0.025mg/ml.3. Continuous application of0.05mg/ml Gastrodin sustain canobviously enhance Schwann cells proliferation and differentiation, as well asNGF expression.