MiR-30a-3p Inhibits Tumor Prolifer-ation, Invasiveness And Metastasis And Is Downregulated In HCC

Background/aims:MicroRNAs (miRNAs) are small non-coding RNAs that regulate physiological and pathological processes by suppressing target gene expression. Altered expression of miR-30a-3p has been demonstrated in several cancers. However, little about how miR-30a-3p functions in these cancers has been reported, and the role of miR-30a-3p in hepatocellular carcinoma (HCC) is unknown. The purpose of this study was to identify the role and underlying molecular mechanism of action of miR-30a-3p in HCC.Methods:A total of110HCC patients, primarily treated by surgical removal of tumors, were involved in the study. HCC cell line Bel-7402was selected to characterize the function of miR-30a-3p in vitro. Results:Our results showed that in83.6%of the110HCC patients, expression of miR-30a-3p was significantly downregulated (P<0.0001) in tumors compared to adjacent normal tissues. In a clinicopathological correlation analysis, downregulation of miR-30a-3p correlated with a significantly higher incidence of portal vein tumor thrombus (PVTT, P=0.009). Moreover, miR-30a-3p markedly inhibited the invasion and migration of Bel-7402HCC cells in vitro. Furthermore, miR-30a-3p overexpression had an inhibitory effect on cell proliferation, induced apoptosis and increased arrest of cells in the S phase. We further demonstrated that miR-30a-3p regulates HCC cell function by a mechanism involving reduced vimentin and MMP3expression and restoration of E-cadherin expression.Conclusions:our data suggest that miR-30a-3p is downregulated in HCC and acts as a tumor suppressor in vitro. Regulation of vimentin, E-cadherin and MMP3by miR-30a-3p suggests a useful therapeutic strategy for tumors with reduced miR-30a-3p expression.