| Objective: To study real-time real time-PCR detection according to protein, Nestinprotein gene expression level in facial nerve repair, discuss the TGF-beta3under theinduction of dental pulp stem cells to repair facial nerve is damaged. Methods: ChooseNew Zealand white rabbit animal model, artificial preparation of specimens of rabbitfacial nerve injury, choose contrast specimens of normal side nerve, were randomlydivided into group two big group in January and march, with dental pulp stem cells andregenerative chamber TGF-beta3repair, rabbit face nerve broken samples, application ofreal-time fluorescent quantitative PCR to detect specific protein markers according tonerve growth and Nestin protein gene expression levels. Results: According to protein andNestin protein in rabbit broken end nerve are specific expression, which according toprotein group of gene mRNA expression level in January mRNA gene expression level inthe march group, significant difference statistically significant (P <0.05); Nestin proteinin a month mRNA in the three months group of low level of gene expression, significantdifference statistically significant (P <0.05); Through the regeneration of dental pulp stemcells and TGF-beta3room repair, injury of facial nerve regeneration. Conclusion: Byfluorescence quantitative PCR detection of dental pulp stem cells and TGF-beta3afterthe repair of injury of facial nerve and nerve growth specific protein markers according toNestin proteins, gene expression at mRNA level has the specific expression, provedthrough the TGF-beta3entrainment, dental pulp stem cells can be directed todifferentiate into neural stem cells to repair facial nerve, facial nerve injury in the clinicalsetting will provide a new approach at the molecular level. |
