Pathological&Physiology Change And Imaging Findings Of Glial Scar After Spinal Cord Injury In Rats

objective: To studies the pathological physiological of glial scar in rat and the changingrule of the imaging changes after spinal cord injury.methods:80male SD rats were randomlydivided into experimental group and control group,40each, the experimental group usedimproved Allen ’s weight fall made mould method; Control group only open the lamina,exposing the spinal cord. Each group at1,3,5,7,14,28,42and56day do MRI scan andSampling the tissue in the damaged region. Use the ELISA method to detect chondrointinsulfate proteoglycans, Immunofluorescence detection of GFAP and NF-200, to observe theformation of glial scar. Results: the content of CSPGs in experimental group at each time point（18.59±0.432~36.82±0.476）was obviously higher than that of control group（7.86±0.371~7.93±0.364，p<0.01）, the difference was statistically significant.Immunofluorescence detection NF200and GFAP find that: GFAP expression ofexperimentalgroup were higher than control group at each time point. In the control group didnot see the glial scar formation. MRI scan found after SCI1day-1weeks the spinal cordwere edema and hemorrhage. Spinal cord were edema, hemorrhage,2weeks to7weeksafter SCI spinal diameter decreased gradually and forms abnormally high signal with theboundary clear shadow. conclusion: Glial scar after SCI is a dynamic change and Graduallystable in the4weeks after injury. Mri scan especially T2WI is important in the diagnosis ofspinal cord injury and The severity of the injury...