| Objective：To explore the effect of the combination therapy of ketotifen and simvastatinon ox-LDL,hs-CRP and Tryptase in atherosclerosis rat serum.Methods：Forty male Wistar rats were randomly divided into four groups, group A wasthe model group,group B ketotifen (ket) interventioned group,group C simvastatininterventioned group, group D ket with simvastatin interventioned group.The rats were fedwith high fat diet and injected with vitamin D3to establish the atherosclerotic animalmodels,then1group B were given mast cell (M C) membrane stability agent ketotifen andthe group C used simvastatin and the group D given two drugs convention. After twelveweeks,todetect serum inflammatory factors and extracte aortic arch which were used in morphologyand immunohistochemical inspection.Results：1. The levels of serum ox-LDL in group D was significantly lower than the other threegroups(p <0.01).2. The levels of serum hs-CRP and Tryptasein in group D was significantly lower thanthe other three groups(p <0.01),and the levels of group B was below group C (p <0.01).3. HE staining:atherosclerotic pathological changes of group D was the lightest tocompare with the other three groups.4. The number of MC and degranulation MC in aorta atherosclerotic was observed whenimmunohistochemical staining:group A, C, B and D showed a trend of graduallydecline(p<0.05). Conclusions：The combination intervention of ketotifen and simvastatin reduced ox-LDL,hs CRPand Tryptase levels,reduced MC around atherosclerotic lesions,and inhibited MCdegranulation, which prompted ketotifen synergy simvastatin increase plaque stability. |
