Apoptosis Effects And Mechanism Of Liver X Receptors Angonist T0901317on Acute Lung Injury

Objective: To observe the effect of liver X receptor agonists T0901317on the acute lunginjury, and the expression of Apoptosis inhibitor6(Api6), to explore the possiblemechanisms of Liver X receptors on apoptosis of acute lung injury.Methods:54male SD rats were divided randomly into the following groups: normalcontrol group(NC group), LPS process group(L group) and LPS+T0901317processgroup(LT group). Acute lung injury model was induced with lipopolysaccharide (LPS),while NC group was injected with equivalent normal saline. Rats were sacrificed and lungtissue was taken at1h,4h,8h after intravenous injection of LPS or normal saline. Arterialblood gas and lung wet/dry weight ratio were analyzed, the pathomorphology change oflung tissue was evaluated by HE-stained lung sections. The expression of Api6proteinwere detected by Western-blot. The amount of survival of macrophage were observed bydetecting the expression of CD68in rats lung tissues and cell apoptosis was tested byTUNEL.Results: Compared with NC group, the ratio of lung wet/dry weight increasedsignificantly in L group (P<0.05), PaO2was significantly reduced(P<0.05); Lung tissuepathology morphlogy displayed interval broadening, pulmonary capillaries filled with gore,tissue edema, inflammatoty cell increased together, while LT group damage wassignificantly reduced, compared with L group. The expression of CD68was detected byimmunohistochemistry (IHC) and the cells apoptosis by TUNEL, Compared with NCgroup, the expression of CD68in L group was significantly reduced(P﹤0.05), apoptoticindex(AI) obviously increased (P﹤0.05); While compared with L group, theexpression of CD68in LT group was significantly increased(P﹤0.05), and AI wasobviously reduced(P﹤0.05). The expression of Api6protein in L group and LT groupwere significantly lower than that in NC group (P<0.05); While compared with L groupthe expression of Api6protein in the LT group was up-regulated significantly(P<0.05).Conclusion:1.The expression of Api6in the LPS group was significantlydown-regulated.2. The expression of Api6could be up-regulated by TO9O1317, and lunginjury could be relieved, the mechanism might be that Api6improvesmacrophage apoptosis.