The Research On Therapeutic Effection And Expression Of Ulinastatin Acted On Urinary SIRS And Renal Tissue From Pigs

Objective:To observe the therapeutic effect of ulinastatin injection on urine SIRS from pig, andfurther to discuss the effection on nuclear factor urinary-derived SIRS kidney tissue(NF-B) by ulinastatin injection.Method.48pigs were randomly divided into3groups which contained sham operation group,model group and ulinastatin treatment group, and the latter two groups were dividedinto subgroups that pigs were operated in4、6、12and24hours. Related indexes ofrenal function (serum creatinine, blood urea nitrogen, serum Cr BUN microglobulinβ2-MG) were tested by automatic biochemical analyzer. Renal tissue NF-κ B weredetected respectively from diffierent molecular levels by Western blot、SP andRT-PCR, and morphological changes in renal tissue were observed by microscope andtransmission electron microscope.Result.SIRS group and the control group of12hours,24hours of creatinine, with statisticalsignificance (P<0.05), SIRS group and the control group of6hours,12hours,24hours of Bun, β2-MG, were statistically significant (P<0.01). With the modeling time,SIRS group of animal serum Cr, Bun, β2-MG increased, Cr in postoperative24hoursreached (122±11.87) Bun μ mol\/L, after24hours to10.05±0.65mmol\/L, β2-MGafter12hours and reached the maximum value of4.14±0.38mg\/L. Ulinastatinserum Cr, Ding Group Bun at the24h time point, β2-MG in12hours and24hoursthan in the SIRS group decreased, the difference was statistically significant. The control group animal operation after Cr, Bun, β2-MG increased slightly, butcompared with the preoperative, no significant difference (P>0.05).0hours beforeoperation,3animal groups, Bun, Cr β2-MG, no statistical significance (P>0.05). Todetect the expression of RT-PCR in renal tissue of NF-κ B p65mRNA: the controlgroup and the comparison between the groups, no significant difference (P>0.05).Expression of ulinastatin treatment group than in model group of NF-κ B p65mRNAafter12h was significantly decreased (P<0.01); expression of SIRS in renal tissue ofNF-κ B p65mRNA group was significantly higher than that of Sham group (P<0.01),24h reaches the maximum value. The expression of Western blot protein: theexpression of NF-κ B protein SIRS of porcine kidney tissue in model group wassignificantly higher than that in sham operation group (P<0.01),24h reaches themaximum value; expression of ulinastatin in the treatment group than the controlgroup, the protein in the postoperative12h was significantly decreased (P<0.01).Immunohistochemical detection of protein: the expression of kidney tissue in modelgroup was significantly higher than that in sham operation group (P<0.01),24hreaches the maximum value; expression of ulinastatin in the treatment group than thecontrol group, the protein in the postoperative12h was significantly decreased(P<0.01). Electron microscopic observation of renal tissue structure of control grouphad no obvious change; group SIRS with the time prolonging, glomerular podocytecell fusion, glomerular endothelial and mesangial cells proliferation and edema,mitochondrial cristae decreased, structural derangement, vacuolization, has broken ormissing, even disintegration; pathological lesion of renal tissue in UTI groupobviously reduce to a lesser extent, glomerular blood sConclusion:Ulinastatin, which may restrain NF-κB’s synthesis and secretion to alleviate thepathological lesion of renal tissue and further to improve renal function, ownedpreferable therapeutic effection on urinary-derived SIRS.