Expression And Clinical Significance Of Regulaotry T Cell In Patients With Tuberculous And Malignant Pleural Effusion

Objective Regulatory T cells （Treg） are a group of T cell subsets having the uniqueimmune function. Treg have an inhibitory effect of restraint or infliction on immuneeffecter cells, through cell contact and secretion of cytokines and play an importantrole in regulation of a variety of immune diseases. Treg become an important part ofresearch in recent years. The more Treg can restrain autoimmune diseases,ease offinflammation and amplify the development of tumor promotion. To investigate theperipheral blood and pleural effusions expression of CD4⁺CD25⁺CD127^lowTreg cellby flow cytometry in patients with tuberculous and malignant pleural effusions andexplore its clinical significance．Methods Twenty samples of tuberculous and malignant pleural effusions patientswere collected and detected the peripheral blood and pleural effusions expression byThree-color direct immune fluorescence labeling method and flow cytometry forCD4⁺CD25⁺CD127^low regulator T cell with20healthy persons as control group.Analysis of the relationship between Treg and survival time of malignant pleuraleffusion patients. SPSS18.0statistical software is used for all data processing. Thepaired t test is used to contrast significant differences of Treg. Analysis of therelationship between Treg and survival time of malignant pleural effusion patientsusing Kaplan-Meier survival curves.Results The percentages of CD4⁺CD25⁺CD127^lowregulatory T cells in peripheralblood in patients with tuberculous and malignant pleural effusions were7.88%±2.16%,10.46%±1.37%,which were much higher than the healthy group4.74%±0.74%（P<0.05）. The percentages of CD4⁺CD25⁺CD127^lowregulatory T cells in pleural effusions in patients with tuberculous and malignant pleural effusionswere8.45%±1.39%，18.98%±5.24%,which were higher than the percentages inperipheral blood of the same groups and the healthy group（P<0.05）. Thepercentages of CD4⁺CD25⁺CD127^lowregulatory T cells in pleural effusions inpatients with malignant pleural were much higher than the percentage in pleuraleffusions in patients with tuberculous. The percentages of CD4⁺CD25⁺CD127^highregulatory T cells in peripheral blood in patients with tuberculous and malignantpleural effusions were much higher than the healthy group（P<0.05）. The lowerexpression of Treg in MPE group had significantly longer overall survival time thanthe higher expression of Treg in MPE group，the median survival time were163daysand112days （P<0.05）.Conclusion The elevated proportion expression of CD4⁺CD25⁺CD127^lowregulatory T cells in peripheral blood and pleural effusions in patients withtuberculous and malignant pleural effusions suggest that regulatory T cell is involvedin the occurrence and development of pleural effusions by regulate immunity,effecting the outcome and prognosis of disease, and the variation of regulator T cellin pleural effusions may be used to be an auxiliary indicator of the diagnosis oftuberculous and malignant pleural effusions.