Research On Inhibit The STAT3and AKT Signaling Pathways Intervention Rabbit Atherosclerosis By Resveratrol

[Objective]To explore the effect of resveratrol on signal transduction mechanism in atherosclerosis model rabbit.[Methods] Fifty-seven males Japan white rabbit randomly divided into seven groups(9rabbits for group A to E,6rabbits for group F and G):group A(normal control)fed with normal feedstuff, group B(20weeks model) fed with high cholesterol feedstuff, resveratrol intervention groupC,D,E,F(after fed20weeks high cholesterol feedstuff treat with resveratrol5mg/kg·d,15mg/kg·d,45mg/kg·d,80mg/kg·d), resveratrol prevent group G(treat with resveratrol80mg/kg·d as well as fed high cholesterol feedstuff). The seven groups are feeding32weeks to be put to death. The experimental animals were sacrificed32weekend and extraction of serum, separate out heart, aorta Specimens.Observation of the gross specimen; heart and aorta specimens, part of the dehydration is fixed, the production organization chip to HE dyed and determination caspase-3expression by immunohistochemical; The artery organization extraction tissue protein, used for protein immune imprinting western blotting test organization inflammation in signaling pathways STAT3and the AKT of the change.[Results]1.20-week model group (B group) the naked eye view:the gross specimen rabbit on the surface of the heart there is a lot of fat tissue coverage, The poor permeability of the aorta; resveratrol prevention group (G group) naked eye view:the gross specimen rabbit surface of the heart have a veryless fat tissue coverage, aortic permeability is good. 2.The rabbits aortic wall anatomy face concept::Group A artery union without atherosclerotic plaque formation, B group had serious lipid plaque, fatpattern formation, in line with the lesions of atherosclerosis. GroupC, D, E group F artery atherosclerotic lesions was slightly reduced; the group C and D as effective as group E, F was significantly; group G than in normal group still lipid plaque formation, but lower than resveratrol in the intervention group (C,D, E, F group) has been reduced.3.Signaling pathway:Group B expression of inflammatory signaling pathway, STAT3and AKT phosphorylation than group A significantly activated (P<0.05); group C and D may be due to the dose low to inhibit the role of key signaling pathways of STAT3phosphorylation of (p>0.05); group E by inhibiting STAT3signaling pathway phosphorylation (p<0.05); group F and G can significantly inhibit STAT3and AKT signaling pathway phosphorylation (p<0.05).4.The positive rate of expression of the apoptotic factors caspase-3:Group A, few caspase-3expression, caspase-3in group B significantly upregulated, groupC, D, E and group F can reduce the expression of caspase-3(p<0.01), Resveratrol prevention group compared to20weeks model group can significantly reduce the expression of caspase-3(p<0.01)[Conclusion] This study suggests that resveratrol have to reduce atherosclerotic plaque progression, anti-atherosclerotic role in the prevention of arterial atherosclerosis; possible mechanism of anti-inflammatory.anti-proliferative, anti-apoptotic. By inhibiting STAT3and AKT phosphorylation of signaling pathways, play an anti-inflammatory,anti-proliferative effects, and the other by inhibiting the apoptotic factors caspase-3expression, played the anti-apoptotic effects, anti-inflammatory, anti-proliferative, anti-apoptotic effect was dose-effect relationship., Resveratrol prevention group suggesting that there is prevention of arterial atherosclerosis.