Function And Gene Expression Of The Active Effux Transporters In Drug-resistant Candida Albicans

[Objective] To investigate the function and gene expression of active efflux transporters in drug resistant candida albicans.[Methods] The broth microdilution method was performed to determ ined the minimal inhibitory concentration of Baicalin and Fluconazole to candida albicans of ATCC32354. clinical and susceptible isolates and clinical isolates with resistance to fluconazole. We compared the eflux of Rhodamine6G between candida albicans of ATCC32354. clinical and susceptible isolates and clinical isolates with resistance to fluconazole, and screened out the resistant strains with significantly increased efflux of Rhodamine6G. Reverse transcriptase polymerase chain reaction(RT-PCR)was used to measure the mRNA levels of active eflux gene CDRl and CDR2.[Results]The eflux of Rhodamine6G was sign ificantly increased in fluconazole resistant strains when glucose was added, and the gene expressions of CDR1and CDR2were also obviously increased, compared with those in the ATCC32354and sensitive strains.[Conclusions]The excessive expression of active eflux pump gene is related to the resistance to fluconazole in Candida albicans. The measurement of Rhodamine6G eflUX is a useful method for the identification of drug resistant strains induced by the excessive expression of active efiux pump.