Study On Design, Syntheses And Properities Of Functional Complexes Based On2-OH(5-Cl-sal)pn Schiff Base Ligand

Schiff bases mainly refers to a class of stable imine connected on the nitrogen atom with an alkyl or aryl. The Schiff base is usually synthesesed by the condensation of an amine and an active carbonyl group. Schiff base compound can be flexible selection for various different types of substituted amines and carbonyl compounds obtained by a condensation reaction, thus becoming a very important organic ligand in organic syntheses. The ligand compound with Schiff structure can form stable complexes with many metal ions, and promising applications of these complexes in catalysis, medicine, analytical chemistry and optical, electrical, magnetic functional materials and other aspects. Therefore, in the developing process of chemical, the Schiff base and its metal complexes has played a very important role, attached great importance by scientists. So far, domestic and foreign scholars in this field of research is in the ascendant, and constant innovation.Obeject:To design and synthesize a new Shiff base and its metal complexes of Zn, Ni、Cu, study the optical,biological,mangnetic nature of the metal complexes, do screening for the discovery of new metal complexes with Shiff base ligands which have good biological fluorescent properties.Methods:According to a previously reported procedure, a new type of aromatic Schiff base were designed and synthesized, and we try to use a variety of methods to culture crystal of metal complex. Structure was characterized by the micro melting point apparatus, infrared spectroscopy, X-ray single crystal diffraction. Fluorescence spectroscopy, automatic inverted fluorescence microscope was used in the comprehensive, in-depth study about its fluorescence properties in solution and in cell. Ultraviolet spectroscopy, gel electrophoresis used to study the interaction of the complex with DNA; magnetic properties of complex was studied by Mpms-7SQUID-type magnetometer.Result:Successfully synthesized the aromatic Schiff ligand2-OH (5-Cl-sal) pn (L1), Liquid diffusion method was used to culture a novel trinuclear zinc metal complex Zn3(2-OH(5-Cl-sal)pn)2(OAc)2(complex1) and trinuclear nickel metal complex [Ni3(2-OH(5-Cl-sal)pn)2(OAc)2(EtOH)2]·H2O (complex2), a novel octanuclear copper metal complex Cu10(2-OH(5-Cl-sal)pn)4(phen)2(H20)6(complex3) was obtained by solvent volatilization,and their crystal structure had been characterized. L1can specific identify Zn2+and produce strong fluorescence in ethanol solvent, and can not interfered by other ions obvious; fast response, good stability; within a certain concentration range, the fluorescence intensity maintain a good linear relationship with the added zinc ion concentration. Solid fluorescence results show that the complex1can display different fluorescent colors upon irradiation its single crystal with the different wavelength of light. L1can produce strong fluorescence by binding intracellular zinc ion. The fluorescence spectrum shows complex2can quench the fluorescence of EB-DNA at a certain degree, Stern-Volmer formula Kapp value as6.53×105M-1. Agarose gel electrophoresis experiments show that:pUC19DNA can be converted from Form I into Form II in the participation of hydrogen peroxide, and with the increasing of complex concentration. Variable temperature magnetic susceptibility results show that the complex3exhibited ferromagnetic and anti ferromagnetic interactions simultaneously.Conclusion:L1as a fluorescent probe of zinc ions exhibited high sensitivity, selectivity, and stability; very fast response; Fast response can be used for real-time monitoring of the concentration of zinc ions; Excellent linear relationship can be used for quantitative detection of the concentration of zinc ions; excellent intracellular fluorescence performance of L1is expected to detect the the zinc ion within biological environment. Flurescence spectroscopy indicates that complex2insert CT-DNA competitively and replace EB by moderate intensity. Agarose gel electrophoresis experiments show that:it caused Medium and weak nuclease action in the participation of hydrogen peroxide, the way of DNA cleavaged by complex is the oxidation cleavage. Magnetic studies show that decanuclear copper metal complex3exhibited erromagnetic and antiferromagnetic interactions simultaneously.