Expression Of Ring Finger Protein180in Gastric Cancer And Its Clinical Significance

Objective:To investigate the expression of RNF180mRNA and protein in human gastric carcinoma tissues; to explore the relationship between the expression of RNF180and clinicopathological features and to estimate the value in the prognosis of gastric cancer.Methods:RNF180gene mRNA in tumor tissue and paired adjacent normal tissue from82gastric cancer patients who were treated in Tianjin Medical University Cancer Hospital from Jan2003to Dec2007were detected by RT-PCR(Reverse Transcription-Polymerase Chain Reaction). The expression of RNF180protein was observed in107gastric cancer patients by immunohistochemical staining. The relationship between expression of RNF180and various clinicopathological factors were evaluated. The role of expression of RNF180in the prognosis of gastric cancer was studied.Results:1. The RNF180gene mRNA expression in gastric tumor tissues were lower than in corresponding para-cancerous tissues in average (P<0.05). Lower RNF180gene mRNA expression was significantly associated with age, histological type, lymph node metastasis and TNM stage (P＜0.05).2. The expression rate of RNF180protein in107gastric carcinoma tissues was44.8%, and91.6%in the corresponding para-cancerous tissues. It was significantly lower than those in the corresponding para-cancerous tissues(P<0.05). Positive RNF180staining was significantly associated with lymph node metastasis and TNM stage(P<0.05).3. There was a significant positive correction between the RNF180gene mRNA expression and the expression rates of RNF180protein in gastric cancer (r=0.217, p=0.025).4. Lymph node metastasis, TNM stage and RNF180expression were associated with the prognosis of gastric cancer(P＜0.05). Multivariate analysis revealed RNF180expression and TNM stage were independently prognostic factors of poor survival (P＜0.05).Conclusions:1. The RNF180gene mRNA and protein expression in gastric tumor tissues were significantly lower than those in corresponding para-cancerous tissues. It suggested that RNF180may be a tumor suppressor gene.2. RNF180expression was an independently prognostic factor in gastric cancer patients after radical surgery. It may be a prognostic factor of gastric cancer.